Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

N Te; J Rodon; R Creve; M Pérez; J Segalés; J Vergara-Alert; A Bensaid

doi:10.1186/s13567-022-01084-3

Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

N Te, J Rodon, R Creve, M Pérez, J Segalés, J Vergara-Alert, A Bensaid

Department of Animal Health and Anatomy

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.

Original language	English
Pages (from-to)	67
Number of pages	1
Journal	Veterinary Research
Volume	53
Issue number	1
DOIs	https://doi.org/10.1186/s13567-022-01084-3
Publication status	Published - 2 Sep 2022

Keywords

Animals
Antiviral Agents
Bronchi
Camelids, New World
Coronavirus Infections/veterinary
Middle East Respiratory Syndrome Coronavirus/physiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s13567-022-01084-3

https://ddd.uab.cat/record/265347

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title = "Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.",

abstract = "Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.",

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AU - Vergara-Alert, J

AU - Bensaid, A

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KW - Middle East Respiratory Syndrome Coronavirus/physiology

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Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

Abstract

Keywords

UN SDGs

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