Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.

N Te, J Rodon, R Creve, M Pérez, J Segalés, J Vergara-Alert, A Bensaid

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.

Original languageEnglish
Pages (from-to)67
Number of pages1
JournalVeterinary Research
Volume53
Issue number1
DOIs
Publication statusPublished - 2 Sep 2022

Keywords

  • Animals
  • Antiviral Agents
  • Bronchi
  • Camelids, New World
  • Coronavirus Infections/veterinary
  • Middle East Respiratory Syndrome Coronavirus/physiology

Fingerprint

Dive into the research topics of 'Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.'. Together they form a unique fingerprint.

Cite this