Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis

I. Latorre, M. De Souza-Galvão, J. Ruiz-Manzano, A. Lacoma, C. Prat, N. Altet, V. Ausina, J. Domínguez

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41 Citations (Scopus)

Abstract

The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright©ERS Journals Ltd 2010.
Original languageEnglish
Pages (from-to)338-342
JournalEuropean Respiratory Journal
Volume35
Issue number2
DOIs
Publication statusPublished - 1 Feb 2010

Keywords

  • Childhood
  • ELISPOT
  • Interferon-γ release assays
  • Latent tuberculosis infection
  • Mycobacterium avium sensitin
  • Non-tuberculous mycobacteria

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