Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis

I. Latorre; M. De Souza-Galvão; J. Ruiz-Manzano; A. Lacoma; C. Prat; N. Altet; V. Ausina; J. Domínguez

doi:10.1183/09031936.00196608

Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis

I. Latorre, M. De Souza-Galvão, J. Ruiz-Manzano, A. Lacoma, C. Prat, N. Altet, V. Ausina, J. Domínguez

Research output: Contribution to journal › Article › Research › peer-review

41 Citations (Scopus)

Abstract

The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright©ERS Journals Ltd 2010.

Original language	English
Pages (from-to)	338-342
Journal	European Respiratory Journal
Volume	35
Issue number	2
DOIs	https://doi.org/10.1183/09031936.00196608
Publication status	Published - 1 Feb 2010

Keywords

Childhood
ELISPOT
Interferon-γ release assays
Latent tuberculosis infection
Mycobacterium avium sensitin
Non-tuberculous mycobacteria

Access to Document

10.1183/09031936.00196608

Fingerprint Dive into the research topics of 'Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis'. Together they form a unique fingerprint.

Cite this

@article{39eb25d0da374a1e8745a9b9ce8599aa,

title = "Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis",

abstract = "The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Gu{\'e}rin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright{\textcopyright}ERS Journals Ltd 2010.",

keywords = "Childhood, ELISPOT, Interferon-γ release assays, Latent tuberculosis infection, Mycobacterium avium sensitin, Non-tuberculous mycobacteria",

author = "I. Latorre and {De Souza-Galv{\~a}o}, M. and J. Ruiz-Manzano and A. Lacoma and C. Prat and N. Altet and V. Ausina and J. Dom{\'i}nguez",

year = "2010",

month = feb,

day = "1",

doi = "10.1183/09031936.00196608",

language = "English",

volume = "35",

pages = "338--342",

number = "2",

}

TY - JOUR

T1 - Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis

AU - Latorre, I.

AU - De Souza-Galvão, M.

AU - Ruiz-Manzano, J.

AU - Lacoma, A.

AU - Prat, C.

AU - Altet, N.

AU - Ausina, V.

AU - Domínguez, J.

PY - 2010/2/1

Y1 - 2010/2/1

N2 - The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright©ERS Journals Ltd 2010.

AB - The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright©ERS Journals Ltd 2010.

KW - Childhood

KW - ELISPOT

KW - Interferon-γ release assays

KW - Latent tuberculosis infection

KW - Mycobacterium avium sensitin

KW - Non-tuberculous mycobacteria

U2 - 10.1183/09031936.00196608

DO - 10.1183/09031936.00196608

M3 - Article

VL - 35

SP - 338

EP - 342

IS - 2

ER -