TY - JOUR
T1 - EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
AU - Roman-Canal, Berta
AU - Tarragona, Jordi
AU - Moiola, Cristian Pablo
AU - Gatius, Sònia
AU - Bonnin, Sarah
AU - Ruiz-Miró, Maria
AU - Sierra, José Enrique
AU - Rufas, Maria
AU - González, Esperanza
AU - Porcel, José M.
AU - Gil-Moreno, Antonio
AU - Falcón-Pérez, Juan M.
AU - Ponomarenko, Julia
AU - Matias-Guiu, Xavier
AU - Colas, Eva
PY - 2019/6/20
Y1 - 2019/6/20
N2 - © 2019 The Author(s). Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
AB - © 2019 The Author(s). Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
KW - Ascitic fluid
KW - Biomarkers
KW - Colon cancer
KW - Colorectal cancer
KW - Diagnostic
KW - Extracellular vesicles
KW - Liquid biopsy
KW - Peritoneal lavage
KW - miRNAs
U2 - https://doi.org/10.1186/s12967-019-1954-8
DO - https://doi.org/10.1186/s12967-019-1954-8
M3 - Article
C2 - 31221189
VL - 17
M1 - 208
ER -