EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer

Berta Roman-Canal, Jordi Tarragona, Cristian Pablo Moiola, Sònia Gatius, Sarah Bonnin, Maria Ruiz-Miró, José Enrique Sierra, Maria Rufas, Esperanza González, José M. Porcel, Antonio Gil-Moreno, Juan M. Falcón-Pérez, Julia Ponomarenko, Xavier Matias-Guiu, Eva Colas

Research output: Contribution to journalArticleResearch

9 Citations (Scopus)

Abstract

© 2019 The Author(s). Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
Original languageEnglish
Article number208
JournalJournal of Translational Medicine
Volume17
DOIs
Publication statusPublished - 20 Jun 2019

Keywords

  • Ascitic fluid
  • Biomarkers
  • Colon cancer
  • Colorectal cancer
  • Diagnostic
  • Extracellular vesicles
  • Liquid biopsy
  • Peritoneal lavage
  • miRNAs

Fingerprint Dive into the research topics of 'EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer'. Together they form a unique fingerprint.

Cite this