Estimated quantity of swine virus genomes based on quantitative PCR analysis in spray-dried porcine plasma samples collected from multiple manufacturing plants.

E Blázquez, J Pujols, J Segalés, C Rodríguez, J Campbell, L Russell, J Polo

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This survey was conducted to estimate the incidence and level of potential viral contamination in commercially collected porcine plasma. Samples of spray dried porcine plasma (SDPP) were collected over a 12- month period from eight spray drying facilities in Spain, England, Northern Ireland, Brazil, Canada, and the United States. In this survey, viral load for several porcine pathogens including SVA, TGEV, PRRSV (EU and US strains), PEDV, PCV-2, SIV, SDCoV and PPV were determined by qPCR. Regression of Ct on TCID 50 of serial diluted stock solution of each virus allowed the estimate of potential viral level in SDPP and unprocessed liquid plasma (using typical solids content of commercially collected porcine plasma). In this survey SVA, TGEV or SDCoV were not detected in any of the SDPP samples. Brazil SDPP samples were free of PRRSV and PEDV. Samples of SDPP from North America primarily contained the PRRSV-US strain while the European samples contained the PRRSV-EU strain (except for one sample from each region containing a relatively low estimated level of the alternative PRRSV strain). Estimated viral level tended to be in the range from <1.0 log 10 TCID 50 to <2.5 log 10 TCID 50. Estimated level of SIV was the exception with a very low incidence rate but higher estimated viral load <3.9 log 10 TCID 50. In summary, the incidence of potential viral contamination in commercially collected porcine plasma was variable and estimated virus level in samples containing viral DNA/RNA was relatively low compared with that occurring at the peak viremia during an infection for all viruses or when considering the minimal infectious dose for each of them.

Original languageEnglish
Article numbere0259613
Pages (from-to)e0259613
JournalPloS one
Volume17
Issue number5 May
DOIs
Publication statusPublished - 1 May 2022

Keywords

  • Animal Feed/analysis
  • Animals
  • Genome, Viral
  • Manufacturing and Industrial Facilities
  • Plasma
  • Porcine Reproductive and Respiratory Syndrome
  • Porcine respiratory and reproductive syndrome virus/genetics
  • RNA, Viral
  • Real-Time Polymerase Chain Reaction
  • Swine
  • Viruses

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