TY - JOUR
T1 - Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: Analytical validation and effects evaluation by single and mixed heavy metal exposure
AU - Franco, Lorena
AU - Romero, Diego
AU - García-Navarro, José A.
AU - Teles, Mariana
AU - Tvarijonaviciute, Asta
PY - 2016/1/15
Y1 - 2016/1/15
N2 - © 2015 Elsevier Ltd. The aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were <. 15%, and recovery were 97%-109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion, the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes.
AB - © 2015 Elsevier Ltd. The aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were <. 15%, and recovery were 97%-109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion, the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes.
KW - Cadmium
KW - Copper
KW - Lead
KW - Mussels
KW - Oxidative stress
KW - Ternary mixture
U2 - 10.1016/j.marpolbul.2015.12.010
DO - 10.1016/j.marpolbul.2015.12.010
M3 - Article
VL - 102
SP - 30
EP - 35
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
IS - 1
ER -