Esterase activity assay by flow injection analysis (FIA)

M. A. Joga, X. Font, M. A. Gordillo, F. Valero

    Research output: Contribution to journalArticleResearchpeer-review

    2 Citations (Scopus)

    Abstract

    An automatic flow injection analysis (FIA) system for on-line determination of esterase activity has been developed. It is based on a colorimetric method using p-nitrophenyl propionate as substrate. The system permits a linear range analysis up to 0.18 U ml-1, although the range can be extended up to 1 U ml-1 without external dilution of the sample. The sampling frequency is of 4 samples per h with a relative standard deviation of 0.9%.
    Original languageEnglish
    Pages (from-to)943-948
    JournalBiotechnology Letters
    Volume23
    DOIs
    Publication statusPublished - 14 Jul 2001

    Keywords

    • Esterase activity
    • FIA
    • p-nitrophenol propionate

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