Establishment and validation of surface model for biodosimetry based on γ-H2AX foci detection

Juan S. López, Mònica Pujol-Canadell, Pedro Puig, Montserrat Ribas, Pablo Carrasco, Gemma Armengol, Joan F. Barquinero*

*Corresponding author for this work

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Abstract

Introduction: In the event of a radiation accident detecting γ-H2AX foci is being accepted as fast method for triage and dose assessment. However, due to their disappearance kinetics, published calibrations have been constructed at specific post-irradiation times. Objectives: To develop a surface, or tridimensional, model to estimate doses at times not included in the calibration analysis, and to validate it. Materials and methods: Calibration data was obtained irradiating peripheral mononucleated cells from one donor with radiation doses ranging from 0 to 3 Gy, and γ -H2AX foci were detected microscopically using a semi-automatic method, at different post-irradiation times from 0.5 to 24 h. For validation, in addition to the above-mentioned donor, blood samples from another donor were also used. Validation was done within the range of doses and post-irradiation times used in the calibration. Results: The calibration data clearly shows that at each analyzed time, the γ-H2AX foci frequency increases as dose increases, and for each dose this frequency decreases with post-irradiation time. The γ-H2AX foci nucleus distribution was clearly overdispersed, for this reason to obtain bidimensional and tridimensional dose-effect relationships no probability distribution was assumed, and linear and non-linear least squares weighted regression was used. In the two validation exercises for most evaluated samples, the 95% confidence limits of the estimated dose were between ±0.5 Gy of the real dose. No major differences were observed between donors. Conclusion: In case of a suspected overexposure to radiation, the surface model here presented allows a correct dose estimation using γ-H2AX foci as biomarker. The advantage of this surface model is that it can be used at any post-irradiation time, in our model between 0.5 and 24 h.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalInternational Journal of Radiation Biology
Volume98
Issue number1
Publication statusPublished - 20 Jan 2022

Keywords

  • BIOLOGICAL DOSIMETRY
  • DICENTRIC CHROMOSOMES
  • DOSE-RESPONSE RELATIONSHIP
  • FLOW-CYTOMETRY
  • INDUCED DNA-DAMAGE
  • IONIZING-RADIATION
  • IRRADIATION
  • PERIPHERAL-BLOOD LYMPHOCYTES
  • STRAND BREAK REPAIR
  • WHOLE-BLOOD
  • biodosimetry
  • gamma-H2AX
  • ionizing radiation
  • surface model

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