Equine platelet-rich plasma cryopreservation

P. Fantini; M. S. Palhares; M. Prades; V. C. Macedo; J. M. Silva Filho; F. O.P. Leme; J. U. Carmona

doi:10.1590/1678-4162-8078

Equine platelet-rich plasma cryopreservation

P. Fantini, M. S. Palhares, M. Prades, V. C. Macedo, J. M. Silva Filho, F. O.P. Leme, J. U. Carmona

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

Equine platelet-rich plasma (PRP) frozen at -196°C in liquid nitrogen using DMSO as a cryoprotectant in two different concentrations (3% and 6%) was evaluated, using platelet morphology and aggregometry as the final parameters. Twelve PRP samples were used in two repetitions. The samples were submitted to slow cooling prior to frozen (-0.07°C/minute) until they reached the temperature of 4-5°C. Platelet cryopreserved in 3% or 6% DMSO, presented similar efficacy when the percentage of activation and platelet aggregation was evaluated.

Original language	English
Pages (from-to)	73-81
Journal	Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
Volume	68
Issue number	1
DOIs	https://doi.org/10.1590/1678-4162-8078
Publication status	Published - 1 Jan 2016

Keywords

Cryopreservation
DMSO
Equine
Platelet-rich plasma (PRP)

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10.1590/1678-4162-8078

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abstract = "Equine platelet-rich plasma (PRP) frozen at -196°C in liquid nitrogen using DMSO as a cryoprotectant in two different concentrations (3% and 6%) was evaluated, using platelet morphology and aggregometry as the final parameters. Twelve PRP samples were used in two repetitions. The samples were submitted to slow cooling prior to frozen (-0.07°C/minute) until they reached the temperature of 4-5°C. Platelet cryopreserved in 3% or 6% DMSO, presented similar efficacy when the percentage of activation and platelet aggregation was evaluated.",

keywords = "Cryopreservation, DMSO, Equine, Platelet-rich plasma (PRP)",

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T1 - Equine platelet-rich plasma cryopreservation

AU - Fantini, P.

AU - Palhares, M. S.

AU - Prades, M.

AU - Macedo, V. C.

AU - Silva Filho, J. M.

AU - Leme, F. O.P.

AU - Carmona, J. U.

PY - 2016/1/1

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N2 - Equine platelet-rich plasma (PRP) frozen at -196°C in liquid nitrogen using DMSO as a cryoprotectant in two different concentrations (3% and 6%) was evaluated, using platelet morphology and aggregometry as the final parameters. Twelve PRP samples were used in two repetitions. The samples were submitted to slow cooling prior to frozen (-0.07°C/minute) until they reached the temperature of 4-5°C. Platelet cryopreserved in 3% or 6% DMSO, presented similar efficacy when the percentage of activation and platelet aggregation was evaluated.

AB - Equine platelet-rich plasma (PRP) frozen at -196°C in liquid nitrogen using DMSO as a cryoprotectant in two different concentrations (3% and 6%) was evaluated, using platelet morphology and aggregometry as the final parameters. Twelve PRP samples were used in two repetitions. The samples were submitted to slow cooling prior to frozen (-0.07°C/minute) until they reached the temperature of 4-5°C. Platelet cryopreserved in 3% or 6% DMSO, presented similar efficacy when the percentage of activation and platelet aggregation was evaluated.

KW - Cryopreservation

KW - DMSO

KW - Equine

KW - Platelet-rich plasma (PRP)

U2 - 10.1590/1678-4162-8078

DO - 10.1590/1678-4162-8078

M3 - Article

VL - 68

SP - 73

EP - 81

JO - Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

JF - Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

SN - 0102-0935

IS - 1

ER -

Equine platelet-rich plasma cryopreservation

Abstract

Keywords

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