Epidermal growth factor inhibits phosphoenolpyruvate carboxykinase gene expression in rat hepatocytes in primary culture

Cristina Fillat, Alfons Valera, Fatima Bosch

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) decreased the basal, and blocked the dibutyryl cyclic AMP (Bt2cAMP)-induced, expression of P-enolpyruvate carboxykinase (GTP) (PEPCK) and tyrosine aminotransferase (TAT) genes in both rat hepatocytes in primary culture and the FTO-2B hepatoma cell line. Treatment of hepatocytes with EGF in combination with phorbol ester (TPA) resulted in an additive decrease of PEPCK mRNA levels. Overnight pretreatment of hepatocytes with TPA, which is known to downregulate protein kinase C, abolished the TPA and reduced the EGF-mediated inhibition of PEPCK gene expression. These results suggested that EGF caused its effect, at least in part, through protein kinase C. © 1993.
Original languageEnglish
Pages (from-to)287-291
JournalFEBS Letters
Volume318
Issue number3
DOIs
Publication statusPublished - 8 Mar 1993

Keywords

  • Epidermal growth factor
  • Gene expression
  • Hepatocyte
  • P-enolpyruvate carboxykinase
  • Phorbol ester

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