Core Messages: This chapter deals with the range of molecular biology methods including PCR-based assays, high-throughput DNA sequence analysis, and the use of real-time PCR in performing studies of skin and environmental community structure and dynamics. It highlights the utility of each molecular biology method in yielding Malassezia epidemiological data, the limitations of culture-based methods, and the possible biases that may influence Malassezia epidemiological studies. As in any disease or health-impacting event, the frequency and patterns of Malassezia-related diseases is examined by descriptive epidemiology. Thus, the chapter includes assessment of patient-related factors such as age, immunological status and gender, and environmental factors such as the geographical area and time of the year that contribute to the analysis of risks from Malassezia-induced or Malassezia -exacerbated disease. Also, a compre-hensive account is given on the Malassezia epidemiology in animals and the factors associated with the animal hosts and their skin microenvironment. © 2010 Springer-Verlag Berlin Heidelberg.
|Title of host publication||Malassezia and the Skin: Science and Clinical Practice|
|Place of Publication||Heidelberg (DE)|
|Number of pages||54|
|Publication status||Published - 1 Dec 2010|