Epidemiological data of different human papillomavirus genotypes in cervical specimens of HIV-1-infected women without history of cervical pathology

Sebastian Videla, Laila Darwich, Maria Paz Cañadas, Roger Paredes, Antoni Tarrats, Eva Castella, Mariona Llatjos, Margarita Bofill, Bonaventura Clotet, Guillem Sirera

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22 Citations (Scopus)

Abstract

Aim: To study the epidemiology of different human papillomavirus (HPV) genotypes in cervical samples of HIV-1-infected women with normal Papanicolau smears. Design: Retrospective analysis of a prospective cohort. Patients and Methods: We selected HIV-1-infected women with 2 consecutive normal Papanicolau smears at baseline and at least 1 baseline and 1 follow-up cervical sample. HPV infection was assessed by second-generation hybrid capture (HC-2) and multiplex polymerase chain reaction (mPCR). HPV genotypes were determined by mPCR. Results: From a cohort of 139 women followed up to 4 years, 93 women meeting the inclusion criteria were analyzed. The mean period between samples was 20 months (range, 6-44 months). HPV baseline prevalence was 63% [59/93; 95% confidence interval (CI), 53% to 73%] using polymerase chain reaction and 41% (38/93; 95% CI, 31 % to 51 %) using HC-2, P = 0.007 (kappa, 0.45; P = 0.001 ). The most prevalent high oncogenic risk genotypes (HR-HPV) were HPV-16 (28%), HPV-33 (18%), HPV-52 (12%), HPV-58 (11%), and HPV-39 (11%). Infection with multiple HPV genotypes was detected in >40% of women. HPV infection persisted at follow-up in 86% (51/59; 95% CI, 77% to 95%) by polymerase chain reaction and 76% (29/38; 95% CI, 62% to 90%) by HC-2. HPV infection persisted in 55% of women with samples available beyond 3 years. The actuarial probabilities of clearance and incidence of HPV infection at 36 months were 16% and 45%, respectively. Conclusions: HPV infection is highly prevalent and persistent among HIV-1-infected women with normal Papanicolau smears. HR-HPV genotypes other than HPV-16 (HPV-33, HPV-52) are frequently detected in HIV-infected women. mPCR provides better surveillance of HPV infection than HC-2 methods. Copyright © 2009 by Lippincott Williams & Wilkins.
Original languageEnglish
Pages (from-to)168-175
JournalJournal of Acquired Immune Deficiency Syndromes
Volume50
DOIs
Publication statusPublished - 1 Feb 2009

Keywords

  • Cervical cytology
  • HIV-HPV
  • Hybrid capture
  • Multiplex PCR
  • Papanicolau

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