Eosinophil-induced neurotoxicity: The role of eosinophil cationic protein/RNase 3

Susanna Navarro, Ester Boix, Claudi M. Cuchillo, M. Victòria Nogués

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23 Citations (Scopus)

Abstract

We analyze the effect of ECP on primary cultures of cerebellar granule cells (CGCs) and astrocytes in an effort to understand the role of ECP in the eosinophil-induced neurotoxicity. We have shown that ECP induces dose-dependent cell death in both CGCs and astrocytes. The effect of ECP action on cell morphology is consistent with apoptosis for both cell types. The apoptotic mechanism involves ECP binding on the cell surface and an increase in the free cytosolic Ca2+ concentration. It is associated with the activation of caspase-3, -8 and -9, processes that are also involved in the apoptosis induced either by stroke or other neurodegenerative conditions. Our results open new insights to clarify the neurotoxic effects associated to ECP in the hypereosinophilic syndrome. © 2010 Elsevier B.V.
Original languageEnglish
Pages (from-to)60-70
JournalJournal of Neuroimmunology
Volume227
DOIs
Publication statusPublished - 1 Oct 2010

Keywords

  • Apoptosis
  • Caspase activity
  • Eosinophil cationic protein
  • Membrane permeability
  • Neurotoxicity
  • Protein aggregation

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