Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase

Susan Hartwig Duarte, Gonzalo Lázaro del Peso Hernández, Albert Canet, Maria Dolors Benaiges, Francisco Maugeri, Francisco Valero

    Research output: Contribution to journalArticleResearchpeer-review

    46 Citations (Scopus)

    Abstract

    © 2015 Elsevier Ltd. The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
    Original languageEnglish
    Pages (from-to)175-180
    JournalBioresource Technology
    Volume183
    DOIs
    Publication statusPublished - 1 May 2015

    Keywords

    • Biodiesel
    • Rhizopus oryzae lipase
    • Single cell oil

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