Enteric neuron density correlates with clinical features of severe gut dysmotility

Elisa Boschetti, Carolina Malagelada, Anna Accarino, Juan R Malagelada, Rosanna F Cogliandro, Alessandra Gori, Elena Bonora, Fiorella Giancola, Francesca Bianco, Vitaliano Tugnoli, Paolo Clavenzani, Fernando Azpiroz, Vincenzo Stanghellini, Catia Sternini, Roberto De Giorgio

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Gastrointestinal (GI) symptoms can originate from severe dysmotility due to enteric neuropathies. Current methods used to demonstrate enteric neuropathies are based mainly on classic qualitative histopathological/immunohistochemical evaluation. This study was designed to identify an objective morphometric method for paraffin-embedded tissue samples to quantify the interganglionic distance between neighboring myenteric ganglia immunoreactive for neuron-specific enolase, as well as the number of myenteric and submucosal neuronal cell bodies/ganglion in jejunal specimens of patients with severe GI dysmotility. Jejunal full-thickness biopsies were collected from 32 patients (22 females; 16-77 yr) with well-characterized severe dysmotility and 8 controls (4 females; 47-73 yr). A symptom questionnaire was filled before surgery. Mann-Whitney U test, Kruskal-Wallis coupled with Dunn's posttest and nonparametric linear regression tests were used for analyzing morphometric data and clinical correlations, respectively. Compared with controls, patients with severe dysmotility exhibited a significant increase in myenteric interganglionic distance (P = 0.0005) along with a decrease in the number of myenteric (P < 0.00001) and submucosal (P < 0.0004) neurons. A 50% reduction in the number of submucosal and myenteric neurons correlated with an increased interganglionic distance and severity of dysmotility. Our study proposes a relatively simple tool that can be applied for quantitative evaluation of paraffin sections from patients with severe dysmotility. The finding of an increased interganglionic distance may aid diagnosis and limit the direct quantitative analysis of neurons per ganglion in patients with an interganglionic distance within the control range.NEW & NOTEWORTHY Enteric neuropathies are challenging conditions characterized by a severe impairment of gut physiology, including motility. An accurate, unambiguous assessment of enteric neurons provided by quantitative analysis of routine paraffin sections may help to define neuropathy-related gut dysmotility. We showed that patients with severe gut dysmotility exhibited an increased interganglionic distance associated with a decreased number of myenteric and submucosal neurons, which correlated with symptoms and clinical manifestations of deranged intestinal motility.

Original languageEnglish
Pages (from-to)G793-G801
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume317
Issue number6
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Correlation of Data
  • Female
  • Gastrointestinal Motility/physiology
  • Humans
  • Immunohistochemistry
  • Intestinal Diseases/immunology
  • Intestines/innervation
  • Male
  • Middle Aged
  • Myenteric Plexus/immunology
  • Nerve Tissue Proteins/analysis
  • Specimen Handling/methods
  • Submucous Plexus/immunology

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