TY - JOUR
T1 - Enhancing Localized Pesticide Action through Plant Foliage by Silver-Cellulose Hybrid Patches
AU - Alonso-Díaz, Alejandro
AU - Floriach-Clark, Jordi
AU - Fuentes, Judit
AU - Capellades, Montserrat
AU - Coll, Núria S.
AU - Laromaine, Anna
PY - 2019/2/11
Y1 - 2019/2/11
N2 - Copyright © 2019 American Chemical Society. Efficacy and efficiency of pesticide application in the field through the foliage still face many challenges. There exists a mismatch between the hydrophobic character of the leaf and the active molecule, low dispersion of the pesticides on the leaves' surface, runoff loss, and rolling down of the active molecules to the field, decreasing their efficacy and increasing their accumulation to the soil. We produced bacterial cellulose-silver nanoparticles (BC-AgNPs) hybrid patches by in situ thermal reduction under microwave irradiation in a scalable manner and obtaining AgNPs strongly anchored to the BC. Those hybrids increase the interaction of the pesticide (AgNPs) with the foliage and avoids runoff loss and rolling down of the nanoparticles. The positive antibacterial and antifungal properties were assessed in vitro against the bacteria Escherichia coli and two agro-economically relevant pathogens: the bacterium Pseudomonas syringae and the fungus Botrytis cinerea. We showed in vivo inhibition of the infection in Nicotiana benthamiana and tomato leaves, as proven by the suppression of the expression of defense molecular markers and reactive oxygen species production. The hydrogel-like character of the bacterial cellulose matrix increases the adherence to the foliage of the patches.
AB - Copyright © 2019 American Chemical Society. Efficacy and efficiency of pesticide application in the field through the foliage still face many challenges. There exists a mismatch between the hydrophobic character of the leaf and the active molecule, low dispersion of the pesticides on the leaves' surface, runoff loss, and rolling down of the active molecules to the field, decreasing their efficacy and increasing their accumulation to the soil. We produced bacterial cellulose-silver nanoparticles (BC-AgNPs) hybrid patches by in situ thermal reduction under microwave irradiation in a scalable manner and obtaining AgNPs strongly anchored to the BC. Those hybrids increase the interaction of the pesticide (AgNPs) with the foliage and avoids runoff loss and rolling down of the nanoparticles. The positive antibacterial and antifungal properties were assessed in vitro against the bacteria Escherichia coli and two agro-economically relevant pathogens: the bacterium Pseudomonas syringae and the fungus Botrytis cinerea. We showed in vivo inhibition of the infection in Nicotiana benthamiana and tomato leaves, as proven by the suppression of the expression of defense molecular markers and reactive oxygen species production. The hydrogel-like character of the bacterial cellulose matrix increases the adherence to the foliage of the patches.
KW - ANTIBACTERIAL PROPERTIES
KW - ANTIMICROBIAL ACTIVITY
KW - COMPOSITES
KW - FILM
KW - GREEN SYNTHESIS
KW - IMPREGNATED BACTERIAL CELLULOSE
KW - IONS
KW - NANOCOMPOSITES
KW - NANOFIBER
KW - NANOPARTICLES
KW - Nicotiana benthamiana
KW - bacterial cellulose
KW - preventing infection
KW - pseudomonas
KW - silver nanoparticles
UR - https://ddd.uab.cat/record/205853
UR - http://www.mendeley.com/research/enhancing-localized-pesticide-action-through-plant-foliage-silvercellulose-hybrid-patches
U2 - https://doi.org/10.1021/acsbiomaterials.8b01171
DO - https://doi.org/10.1021/acsbiomaterials.8b01171
M3 - Article
VL - 5
SP - 413
EP - 419
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 2
ER -