Enhanced production of p<inf>L</inf>-controlled recombinant proteins and plasmid stability in Escherichia coli RecA<sup>+</sup> strains

A. Benito, M. Vidal, A. Villaverde

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

Overexpression of pL-controlled foot-and-mouth disease virus recombinant proteins was studied in Escherichia coli RecA+ strains and in a recA mutant. Higher protein yield and extractable plasmid DNA amounts were found in wild type cells, in absence of detectable RecA proteolytic activity. Minor but still significant differences in pBR322 DNA amounts were also detected between RecA+ and its recA13 and lexA1 derivatives. These data should be seriously considered to select expression systems and to design production processes for recombinant proteins, specially if they are expected to be toxic for Escherichia coli cells. © 1993.
Original languageEnglish
Pages (from-to)299-306
JournalJournal of Biotechnology
Volume29
Issue number3
DOIs
Publication statusPublished - 1 Jan 1993

Keywords

  • Escherichia coli
  • FMDV
  • Plasmid stability
  • RecA
  • Recombinant protein

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