Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona

Candela Fernández-Naval; Maider Arando; Mateu Espasa; Andrés Antón; Miguel Fernández-Huerta; Aroa Silgado; Inmaculada Jimenez; Ana M. Villatoro; Juan J. González-López; Judit Serra-Pladevall; Elena Sulleiro; Tomàs Pumarola; Martí Vall-Mayans; Juliana Esperalba

doi:10.2217/fmb-2019-0123

Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona

Candela Fernández-Naval, Maider Arando, Mateu Espasa, Andrés Antón, Miguel Fernández-Huerta, Aroa Silgado, Inmaculada Jimenez, Ana M. Villatoro, Juan J. González-López, Judit Serra-Pladevall, Elena Sulleiro, Tomàs Pumarola, Martí Vall-Mayans, Juliana Esperalba

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research

5 Citations (Scopus)

Abstract

© 2019 Future Medicine Ltd. Aim: To describe the molecular types of Treponema pallidum and the proportion of macrolide and tetracycline resistance mutations in Barcelona. Materials & methods: Molecular type was determined using the Enhanced-CDC Typing system and antibiotic resistance was determined by sequencing the 23S and 16S rRNA genes. Results: A total of 183 patients were enrolled and 213 specimens (99 ulcers, 114 bloods) were collected. Sixty-Two (70.5%) of 88 ulcers and 0 (0%) of bloods T. pallidum-DNA containing samples were fully typed. Up to 21 different strain types were identified (14d/g in 27.4%; 14f/g in 14.5%). Macrolide resistance mutations were present in 95% and tetracycline in 0%. Conclusion: Several different strains co-exist in Barcelona with a high proportion of macrolide resistance and absence of tetracycline resistance.

Original language	English
Pages (from-to)	1099-1108
Journal	Future Microbiology
Volume	14
DOIs	https://doi.org/10.2217/fmb-2019-0123
Publication status	Published - 1 Jan 2019

Keywords

ECDCT methodology
macrolide resistance
molecular typing
syphilis
tetracycline resistance
Treponema pallidum

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10.2217/fmb-2019-0123

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Fernández-Naval, C., Arando, M., Espasa, M., Antón, A., Fernández-Huerta, M., Silgado, A., Jimenez, I., Villatoro, A. M., González-López, J. J., Serra-Pladevall, J., Sulleiro, E., Pumarola, T., Vall-Mayans, M., & Esperalba, J. (2019). Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona. Future Microbiology, 14, 1099-1108. https://doi.org/10.2217/fmb-2019-0123

Fernández-Naval, Candela ; Arando, Maider ; Espasa, Mateu ; Antón, Andrés ; Fernández-Huerta, Miguel ; Silgado, Aroa ; Jimenez, Inmaculada ; Villatoro, Ana M. ; González-López, Juan J. ; Serra-Pladevall, Judit ; Sulleiro, Elena ; Pumarola, Tomàs ; Vall-Mayans, Martí ; Esperalba, Juliana. / Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona. In: Future Microbiology. 2019 ; Vol. 14. pp. 1099-1108.

@article{dfebc6e1f68a4ef0b1a48c2d7999d3af,

title = "Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona",

abstract = "{\textcopyright} 2019 Future Medicine Ltd. Aim: To describe the molecular types of Treponema pallidum and the proportion of macrolide and tetracycline resistance mutations in Barcelona. Materials & methods: Molecular type was determined using the Enhanced-CDC Typing system and antibiotic resistance was determined by sequencing the 23S and 16S rRNA genes. Results: A total of 183 patients were enrolled and 213 specimens (99 ulcers, 114 bloods) were collected. Sixty-Two (70.5%) of 88 ulcers and 0 (0%) of bloods T. pallidum-DNA containing samples were fully typed. Up to 21 different strain types were identified (14d/g in 27.4%; 14f/g in 14.5%). Macrolide resistance mutations were present in 95% and tetracycline in 0%. Conclusion: Several different strains co-exist in Barcelona with a high proportion of macrolide resistance and absence of tetracycline resistance.",

keywords = "ECDCT methodology, macrolide resistance, molecular typing, syphilis, tetracycline resistance, Treponema pallidum",

author = "Candela Fern{\'a}ndez-Naval and Maider Arando and Mateu Espasa and Andr{\'e}s Ant{\'o}n and Miguel Fern{\'a}ndez-Huerta and Aroa Silgado and Inmaculada Jimenez and Villatoro, {Ana M.} and Gonz{\'a}lez-L{\'o}pez, {Juan J.} and Judit Serra-Pladevall and Elena Sulleiro and Tom{\`a}s Pumarola and Mart{\'i} Vall-Mayans and Juliana Esperalba",

year = "2019",

month = jan,

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doi = "10.2217/fmb-2019-0123",

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Fernández-Naval, C, Arando, M, Espasa, M, Antón, A, Fernández-Huerta, M, Silgado, A, Jimenez, I, Villatoro, AM, González-López, JJ, Serra-Pladevall, J, Sulleiro, E , Pumarola, T, Vall-Mayans, M & Esperalba, J 2019, 'Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona', Future Microbiology, vol. 14, pp. 1099-1108. https://doi.org/10.2217/fmb-2019-0123

Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona. / Fernández-Naval, Candela; Arando, Maider; Espasa, Mateu; Antón, Andrés; Fernández-Huerta, Miguel; Silgado, Aroa; Jimenez, Inmaculada; Villatoro, Ana M.; González-López, Juan J.; Serra-Pladevall, Judit; Sulleiro, Elena ; Pumarola, Tomàs; Vall-Mayans, Martí; Esperalba, Juliana.

In: Future Microbiology, Vol. 14, 01.01.2019, p. 1099-1108.

Research output: Contribution to journal › Article › Research

TY - JOUR

T1 - Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona

AU - Fernández-Naval, Candela

AU - Arando, Maider

AU - Espasa, Mateu

AU - Antón, Andrés

AU - Fernández-Huerta, Miguel

AU - Silgado, Aroa

AU - Jimenez, Inmaculada

AU - Villatoro, Ana M.

AU - González-López, Juan J.

AU - Serra-Pladevall, Judit

AU - Sulleiro, Elena

AU - Pumarola, Tomàs

AU - Vall-Mayans, Martí

AU - Esperalba, Juliana

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2019 Future Medicine Ltd. Aim: To describe the molecular types of Treponema pallidum and the proportion of macrolide and tetracycline resistance mutations in Barcelona. Materials & methods: Molecular type was determined using the Enhanced-CDC Typing system and antibiotic resistance was determined by sequencing the 23S and 16S rRNA genes. Results: A total of 183 patients were enrolled and 213 specimens (99 ulcers, 114 bloods) were collected. Sixty-Two (70.5%) of 88 ulcers and 0 (0%) of bloods T. pallidum-DNA containing samples were fully typed. Up to 21 different strain types were identified (14d/g in 27.4%; 14f/g in 14.5%). Macrolide resistance mutations were present in 95% and tetracycline in 0%. Conclusion: Several different strains co-exist in Barcelona with a high proportion of macrolide resistance and absence of tetracycline resistance.

AB - © 2019 Future Medicine Ltd. Aim: To describe the molecular types of Treponema pallidum and the proportion of macrolide and tetracycline resistance mutations in Barcelona. Materials & methods: Molecular type was determined using the Enhanced-CDC Typing system and antibiotic resistance was determined by sequencing the 23S and 16S rRNA genes. Results: A total of 183 patients were enrolled and 213 specimens (99 ulcers, 114 bloods) were collected. Sixty-Two (70.5%) of 88 ulcers and 0 (0%) of bloods T. pallidum-DNA containing samples were fully typed. Up to 21 different strain types were identified (14d/g in 27.4%; 14f/g in 14.5%). Macrolide resistance mutations were present in 95% and tetracycline in 0%. Conclusion: Several different strains co-exist in Barcelona with a high proportion of macrolide resistance and absence of tetracycline resistance.

KW - ECDCT methodology

KW - macrolide resistance

KW - molecular typing

KW - syphilis

KW - tetracycline resistance

KW - Treponema pallidum

U2 - 10.2217/fmb-2019-0123

DO - 10.2217/fmb-2019-0123

M3 - Article

C2 - 31512516

VL - 14

SP - 1099

EP - 1108

JO - Future Microbiology

JF - Future Microbiology

SN - 1746-0913

ER -

Enhanced molecular typing and macrolide and tetracycline-resistance mutations of Treponema pallidum in Barcelona

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Keywords

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