Engineering Regulable Escherichia coli β-Galactosidases as Biosensors for Anti-HIV Antibody Detection in Human Sera

Neus Ferrer-Miralles, Jordi X. Feliu, Stéphane Vandevuer, Annette Müller, Joaquin Cabrera-Crespo, Isabelle Ortmans, Frank Hoffmann, Daniel Cazorla, Ursula Rinas, Martine Prévost, Antonio Villaverde

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32 Citations (Scopus)

Abstract

The activity of engineered, peptide-displaying enzymes is modulated by binding to specific anti-peptide antibodies. This new concept of a quantitative antibody detection system allows test kits to be set up for fast diagnosis of infectious diseases. To develop a quick and homogeneous assay for the detection of human immunodeficiency virus (HIV) infection, we have explored two acceptor sites of the bacterial Escherichia coli β-galactosidase for the accommodation of HIV antigenic peptides. Two overlapping epitopes (namely P1 and P2) from the gp41 envelope glycoprotein, contained in different sized peptides, were inserted in the vicinity of the enzyme active site to generate a set of hybrid, enzymatically active β-galactosidases. Regulable enzymes of different responsiveness to monoclonal antibody binding were generated with both acceptor sites tested. These biosensors were also sensitive to immune sera from HIV-infected patients. Modeling data provide insight into the structural modifications in the vicinity of the active site induced by peptide insertion that strongly affect the responsiveness of the engineered proteins through different parameters of their catalytic properties.
Original languageEnglish
Pages (from-to)40087-40095
JournalJournal of Biological Chemistry
Volume276
Issue number43
DOIs
Publication statusPublished - 26 Oct 2001

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