Engineering multifunctional protein nanoparticles by in vitro disassembling and reassembling of heterologous building blocks

Ugutz Unzueta, Naroa Serna, Laura Sánchez-García, Mónica Roldán, Alejandro Sánchez-Chardi, Ramón Mangues, Antonio Villaverde, Esther Vázquez

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

© 2017 IOP Publishing Ltd. The engineering of protein self-assembling at the nanoscale allows the generation of functional and biocompatible materials, which can be produced by easy biological fabrication. The combination of cationic and histidine-rich stretches in fusion proteins promotes oligomerization as stable protein-only regular nanoparticles that are composed by a moderate number of building blocks. Among other applications, these materials are highly appealing as tools in targeted drug delivery once empowered with peptidic ligands of cell surface receptors. In this context, we have dissected here this simple technological platform regarding the controlled disassembling and reassembling of the composing building blocks. By applying high salt and imidazole in combination, nanoparticles are disassembled in a process that is fully reversible upon removal of the disrupting agents. By taking this approach, we accomplish here the in vitro generation of hybrid nanoparticles formed by heterologous building blocks. This fact demonstrates the capability to generate multifunctional and/or multiparatopic or multispecific materials usable in nanomedical applications.
Original languageEnglish
Article number505102
JournalNanotechnology
Volume28
Issue number50
DOIs
Publication statusPublished - 22 Nov 2017

Keywords

  • cell targeting
  • multifunctional nanoparticles
  • recombinant proteins
  • self-assembling
  • viral mimetics

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