Embryo development and sex ratio of in vitro-produced porcine embryos are affected by the energy substrate and hyaluronic acid added to the culture medium

Eva Torner, Eva Bussalleu, M. Dolors Briz, Marc Yeste, Sergi Bonet

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    Abstract

    In the present study, the effects of replacing glucose with pyruvate-lactate and supplementing these in vitro culture (IVC) media with hyaluronic acid (HA) on porcine embryo development and sex ratio were examined. The in vitro-produced (IVP) porcine embryos were cultured in NCSU-23 medium with 0.0, 0.5 or 1.0 mg mL-1 HA, and with either 5.55 mM glucose (IVC-Glu) or pyruvate (0.17 mM)-lactate (2.73 mM) from 0 to 48 h post insemination (h.p.i.) and then with glucose from 48 to 168 h.p.i. (IVC-PL). Those embryos cultured with IVC-PL had significantly higher blastocyst rates (23.7±1.5%) than those cultured with IVC-Glu (14.27±2.75%). At 1.0 mg mL-1, HA tended to skew the sex ratio of blastocysts towards males in those embryos cultured in IVC-PL, and led to a significant decrease in the blastocyst rate compared with embryos cultured in the presence of 0.5 and 0.0 mg mL-1 HA and IVC-Glu (4.28±0.28% vs 11.01±1.42% and 10.14±2.77%, respectively) and IVC-PL (14.37±1.35% vs 20.96±2.85% and 22.99±1.39%, respectively). In contrast, there were no significant differences in the total cell number per blastocyst or in apoptosis rates. In conclusion, pyruvate and lactate were the preferred energy substrates in the early stages of IVP porcine embryos. Moreover, 1.0 mg mL -1 HA significantly decreased the percentage of blastocyst rates in both the IVC-Glu and IVC-PL groups, but only by a preferential loss of female embryos for those cultured in IVC-PL.© CSIRO 2014.
    Original languageEnglish
    Pages (from-to)570-577
    JournalReproduction, Fertility and Development
    Volume26
    Issue number4
    DOIs
    Publication statusPublished - 1 Jan 2014

    Keywords

    • blastocyst
    • glucose
    • hyaluronan
    • pig
    • pyruvate - lactate

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  • Cite this

    Torner, E., Bussalleu, E., Briz, M. D., Yeste, M., & Bonet, S. (2014). Embryo development and sex ratio of in vitro-produced porcine embryos are affected by the energy substrate and hyaluronic acid added to the culture medium. Reproduction, Fertility and Development, 26(4), 570-577. https://doi.org/10.1071/RD13004