Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome

Gucci Jijuan Gu Urban, Mikaela Friedman, Ping Ren, Carina Törn, Malin Fex, Christiane S. Hampe, Åke Lernmark, Ulf Landegren, Masood Kamali-Moghaddam

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

© 2015 Scientific Reports. Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.
Original languageEnglish
Article number11196
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 16 Jun 2015

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