Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Miguel Aller-Pellitero, Joséphine Fremeau, Rosa Villa, Gonzalo Guirado, Boris Lakard, Jean Yves Hihn, F. Javier del Campo

Research output: Contribution to journalArticleResearch

15 Citations (Scopus)


© 2019 Elsevier B.V. Prussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PB-based electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide. This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 μM H2O2 and 54 μM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 μM and 15 μM, respectively. The dynamic ranges of the glucose biosensors obtained at the PB-based electrodes are 0.1–1 mM (amperometric) and 0.025–2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.
Original languageEnglish
Pages (from-to)591-597
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 1 Jul 2019


  • Biosensors
  • Glucose sensing
  • Hydrogen peroxide detection
  • Prussian Blue electrodes
  • Screen-printed electrodes
  • Spectroelectrochemistry


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