Electrochemical magneto immunosensor for the detection of anti-TG2 antibody in celiac disease

Silvina V. Kergaravat, Luis Beltramino, Nidia Garnero, Liliana Trotta, Marta Wagener, Maria Isabel Pividori, Silvia R. Hernandez

Research output: Contribution to journalArticleResearchpeer-review

32 Citations (Scopus)

Abstract

An electrochemical magneto immunosensor for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The immunological reaction is performed on magnetic beads (MBs) as a solid support in which the transglutaminase enzyme (TG2) is covalently immobilized (TG2-MB) and then ATG2 were revealed by an antibody labeled with peroxidase. The electrochemical response of the enzymatic reaction with o-phenilendiamine and H2O2 as substrates by square wave voltammetry was correlated with the ATG2. Graphite-epoxi composite cylindrical electrodes and screen printed electrodes were used as transducers in the immunosensor. A total number of 29 sera from clinically confirmed cases of celiac disease and 19 negative control sera were tested by the electrochemical magneto immunosensor. The data were submitted to the receiver-operating characteristic plot (ROC) analysis which indicated that 16.95 units was the most effective cut-off value (COV) to discriminate correctly between celiac and non-celiac patients. Using this point for prediction, sensitivity was found to be 100%, while specificity was 84%. © 2013 Elsevier B.V.
Original languageEnglish
Pages (from-to)203-209
JournalBiosensors and Bioelectronics
Volume48
DOIs
Publication statusPublished - 15 Oct 2013

Keywords

  • Anti-transglutaminase antibody
  • Celiac disease
  • Electrochemical
  • Magnetic bead
  • Receiver-operating characteristic

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