Electrochemical magneto-actuated biosensor for CD4 count in AIDS diagnosis and monitoring

S. Carinelli, C. Xufré Ballesteros, M. Martí, S. Alegret, M. I. Pividori

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

© 2015 Elsevier B.V. The counting of CD4+ T lymphocytes is a clinical parameter used for AIDS diagnosis and follow-up. As this disease is particularly prevalent in developing countries, simple and affordable CD4 cell counting methods are urgently needed in resource-limited settings. This paper describes an electrochemical magneto-actuated biosensor for CD4 count in whole blood. The CD4+ T lymphocytes were isolated, preconcentrated and labeled from 100μL of whole blood by immunomagnetic separation with magnetic particles modified with antiCD3 antibodies. The captured cells were labeled with a biotinylated antiCD4 antibody, followed by the reaction with the electrochemical reporter streptavidin-peroxidase conjugate. The limit of detection for the CD4 counting magneto-actuated biosensor in whole blood was as low as 44cellsμL-1 while the logistic range was found to be from 89 to 912cellsμL-1, which spans the whole medical interest range for CD4 counts in AIDS patients. The electrochemical detection together with the immunomagnetic separation confers high sensitivity, resulting in a rapid, inexpensive, robust, user-friendly method for CD4 counting. This approach is a promising alternative for the costly standard flow cytometry and suitable as diagnostic tool at decentralized practitioner sites in low resource settings, especially in less developed countries.
Original languageEnglish
Pages (from-to)974-980
JournalBiosensors and Bioelectronics
Volume74
DOIs
Publication statusPublished - 5 Dec 2015

Keywords

  • AIDS
  • CD4 counting
  • CD4<sup>+</sup> T lymphocyte
  • Electrochemical biosensor
  • HIV
  • Immunomagnetic separation
  • Magnetic particle
  • Magneto-electrode

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