Electrochemical investigation of cellular uptake of quantum dots decorated with a proline-rich cell penetrating peptide

Sergio Marín, Sílvia Pujals, Ernest Giralt, Arben Merkoçi

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

The use of square wave voltammetry to monitor the cellular uptake, in HeLa cells, of quantum dots (QD) decorated with sweet arrow peptide (SAP) is reported. A SAP derivative containing an additional N-terminal cysteine residue (C-SAP) was synthesized using the solid-phase method and conjugated to QDs. The obtained results show that QDs-SAP either interact with the extracellular cell membrane matrix or translocate the bilayer. The first situation, membrane adsorption, is probably a transient state before cellular uptake. Both confocal microscopy and SWV results support the detection of this cellular internalization process. The developed electrochemical investigation technique can provide valuable insights into the study of peptide-mediated delivery, as well as the design and development of nanoparticle probes for intracellular imaging, diagnostic, and therapeutic applications. In addition, the described electrochemical interrogation is low cost, is easy to use, and offers future interest for diagnostics including cell analysis. © 2011 American Chemical Society.
Original languageEnglish
Pages (from-to)180-185
JournalBioconjugate Chemistry
Volume22
Issue number2
DOIs
Publication statusPublished - 16 Feb 2011

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