Electrochemical detection of Salmonella using gold nanoparticles

André S. Afonso, Briza Pérez-López, Ronaldo C. Faria, Luiz H.C. Mattoso, Manuela Hernández-Herrero, Artur Xavier Roig-Sagués, Marisa Maltez-da Costa, Arben Merkoçi

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Abstract

A disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) that incorporates a permanent magnet underneath. Salmonella containing samples (i.e. skimmed milk) have been tested by using anti-Salmonella magnetic beads (MBs-pSAb) as capture phase and sandwiching afterwards with AuNPs modified antibodies (sSAb-AuNPs) detected using differential pulse voltammetry (DPV). A detection limit of 143 cellsmL-1 and a linear range from 103 to 106 cellsmL-1 of Salmonella was obtained, with a coefficient of variation of about 2.4%. Recoveries of the sensor by spiking skimmed milk with different quantities of Salmonella of about 83% and 94% for 1.5×103 and 1.5×105 cellsmL-1 were obtained, respectively. This AuNPs detection technology combined with magnetic field application reports a limit of detection lower than the conventional commercial method carried out for comparison purposes in skimmed milk samples. © 2012 Elsevier B.V.
Original languageEnglish
Pages (from-to)121-126
JournalBiosensors and Bioelectronics
Volume40
DOIs
Publication statusPublished - 15 Feb 2013

Keywords

  • Electrochemical detection
  • Gold nanoparticles
  • Label
  • Magneto-immunoassay
  • Salmonella

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    Afonso, A. S., Pérez-López, B., Faria, R. C., Mattoso, L. H. C., Hernández-Herrero, M., Roig-Sagués, A. X., Maltez-da Costa, M., & Merkoçi, A. (2013). Electrochemical detection of Salmonella using gold nanoparticles. Biosensors and Bioelectronics, 40, 121-126. https://doi.org/10.1016/j.bios.2012.06.054