Electrochemical detection of dopamine using streptavidin-coated magnetic particles and carbon nanotube wiring

Zorione Herrasti, Fernando Martínez, Eva Baldrich

    Research output: Contribution to journalArticleResearchpeer-review

    24 Citations (Scopus)

    Abstract

    Magnetic particles (MP) and carbon nanotubes (CNT) have been extensively exploited in biosensor development. It has been recently suggested that combining MP binding of electroactive molecules with CNT wiring of the MP surface could provide novel sensing formats. Here, we demonstrate the validity of this approach using as a model dopamine (DA). As we show, DA can be electrostatically bound and concentrated using commercially available streptavidin-coated MP. Electrochemical detection of MP-bound DA is then accomplished by CNT wiring using cheap and disposable screen printed electrodes of different sizes and materials. The parameters potentially affecting DA binding, CNT wiring and electrochemical detection have been studied and optimized. The resulting assay takes 45 min, displays LOD of 2 nM DA in saline solution, is unaffected by ascorbic acid and uric acid, and can be operated in diluted urine human samples. Our results confirm that CNT wiring allows efficient detection of electroactive species after MP binding and is compatible with the study of complex real samples. © 2014 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)891-898
    JournalSensors and Actuators, B: Chemical
    Volume203
    DOIs
    Publication statusPublished - 1 Jan 2014

    Keywords

    • Carbon nanotubes (CNT)
    • CNT wiring
    • Dopamine electrochemical detection
    • Magnetic particles (MP)
    • Urine real samples

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