Electrochemical detection in vitro and electron transfer mechanism of testosterone using a modified electrode with a cobalt oxide film

Silio Lima Moura, Rafael Rodrigues De Moraes, Marcos Antônio Pereira Dos Santos, Davyson De Lima Moreira, José Arimatéia Dantas Lopes, Maria Isabel Pividori, Valtencir Zucolotto, José Ribeiro Dos Santos Júnior

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6 Citations (Scopus)

Abstract

The voltammetric oxidation of testosterone (4-androsten-17β-ol-3-one) has been studied at an edge plane glassy carbon electrode modified with cobalt oxide. One sensitive and well-defined peak is obtained with a peak potential of 0.50 V vs. SCE attributed to the reduction reaction CoIV/Co III, which is responsible for oxidation of the steroid. A calibration curve was obtained for testosterone, with the measurements performed in triplicate, in the range from 0.33 to 2.00 μM, yielding a correlation coefficient >0.99 and detection limit of 0.16 μM, with relative standard deviations (R.S.D) from 1.33 × 10-5 to 4.00 × 10 -4% with 95% significance (p < 0.05). The modified electrode showed a stable and reproducible response toward the detection of this steroid. Thus, the results showed that the electrode modified with cobalt oxide acts as a promising alternative for simple, cheap and rapid analysis of testosterone. © 2014 Elsevier B.V.
Original languageEnglish
Pages (from-to)469-474
JournalSensors and Actuators, B: Chemical
Volume202
DOIs
Publication statusPublished - 31 Oct 2014

Keywords

  • Antidoping
  • Cobalt oxide
  • Electrochemical sensor
  • Electron transfer
  • Testosterone

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