Electrochemical biosensing of non-electroactive targets using ferrocene-labeled magnetic particles and CNT wiring

Zorione Herrasti, Rosa Olivé-Monllau, Francesc Xavier Muñoz-Pascual, Fernando Martínez, Eva Baldrich

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    5 Citations (Scopus)

    Abstract

    We show that target binding onto ferrocene-modified magnetic microparticles (MP-Fc) promotes physical sheltering of the labels. This can be measured electrochemically by CNT wiring, which enhances ten-fold the signals registered compared to direct detection of the MPs alone. As a proof of concept, detection of detergents and antibodies is accomplished. In these preliminary experiments, random binding of 0.01% Tween 20 onto MP-Fc was detectable both voltametrically and impedimetrically after a 2 min incubation. Furthermore, affinity capture of 4 μg mL−1of biotinylated antibody by streptavidin MP-Fc could be measured in less than 30 min and even in the presence of 1 mg mL−1of BSA. © 2014 The Royal Society of Chemistry.
    Original languageEnglish
    Pages (from-to)1334-1339
    JournalThe Analyst
    Volume139
    Issue number6
    DOIs
    Publication statusPublished - 17 Feb 2014

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