Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

Ester Ballana, Jing Wang, Frédéric Venail, Xavier Estivill, Jean Luc Puel, Maria L. Arbonès, Assumpció Bosch

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms. © 2008 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)134-139
JournalNeuroscience Letters
Volume442
DOIs
Publication statusPublished - 12 Sep 2008

Keywords

  • AAV5 serotype
  • Gene transfer
  • Inner ear
  • Supporting cells

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