Effects of vaccination against paratuberculosis on tuberculosis in goats: diagnostic interferences and cross-protection

Bernat Pérez de Val, Miquel Nofrarías, Sergio López-Soria, Joseba M. Garrido, H. M. Vordermeier, Bernardo Villarreal-Ramos, Maite Martín, Eugenia Puentes, Ramón A. Juste, Mariano Domingo

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21 Citations (Scopus)

Abstract

Background: Most countries carrying out campaigns of bovine tuberculosis (TB) eradication impose a ban on the use of mycobacterial vaccines in cattle. However, vaccination against paratuberculosis (PTB) in goats is often allowed even when its effect on TB diagnosis has not been fully evaluated. To address this issue, goat kids previously vaccinated against PTB were experimentally infected with TB.Results: Evaluation of interferon-γ (IFN-γ) secretion induced by avian and bovine tuberculins (PPD) showed a predominant avian PPD-biased response in the vaccinated group from week 4 post-vaccination onward. Although 60% of the animals were bovine reactors at week 14, avian PPD-biased responses returned at week 16. After challenge with M. caprae, the IFN-γ responses radically changed to show predominant bovine PPD-biased responses from week 18 onward. In addition, cross-reactions with bovine PPD that had been observed in the vaccinated group at week 14 were reduced when using the M. tuberculosis complex-specific antigens ESAT-6/CFP-10 and Rv3615c as new DIVA (differentiation of infected and vaccinated animals) reagents, which further maintained sensitivity post-challenge. Ninety percent of the animals reacted positively to the tuberculin cervical comparative intradermal test performed at 12 weeks post-infection. Furthermore, post-mortem analysis showed reductions in tuberculous lesions and bacterial burden in some vaccinated animals, particularly expressed in terms of the degree of extrapulmonary dissemination of TB infection.Conclusions: Our results suggest a degree of interference of PTB vaccination with current TB diagnostics that can be fully mitigated when using new DIVA reagents. A partial protective effect associated with vaccination was also observed in some vaccinated animals. © 2012 Pérez de Val et al.; licensee BioMed Central Ltd.
Original languageEnglish
Article number191
JournalBMC Veterinary Research
Volume8
DOIs
Publication statusPublished - 16 Oct 2012

Keywords

  • Diagnostic
  • Goat
  • Interferon gamma
  • Paratuberculosis
  • Tuberculosis
  • Vaccine

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