TY - JOUR
T1 - Effects of true-to-life PET nanoplastics using primary human nasal epithelial cells
AU - Annangi, Balasubramanyam
AU - Villacorta, Aliro
AU - Vela, Lourdes
AU - Tavakolpournegari, Alireza
AU - Marcos, Ricard
AU - Hernández, Alba
N1 - A. Villacorta was supported by Ph.D. fellowships from the National Agency for Research and Development (ANID), CONICYT PFCHA/DOCTORADO BECAS CHILE/2020-72210237. L. Vela was supported by Ph.D. fellowships from Fundación Carolina. A. Tavakolpournegari holds a Ph.D. FI fellowship from the Generalitat de Catalunya. A. Hernández was granted an ICREA ACADEMIA Award.
This project (Plasticheal) has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 965196. This work was partially supported by the Spanish Ministry of Science and Innovation [PID2020-116789, RB-C43] and the Generalitat de Catalunya (2021-SGR-00731).
PY - 2023/6
Y1 - 2023/6
N2 - Since inhalation is a relevant exposure route, studies using appropriate micro/nanoplastic (MNPLs) models, representative targeted cells, and relevant biomarkers of effect are required. We have used lab-made polyethylene terephthalate (PET)NPLs obtained from PET plastic water bottles. Human primary nasal epithelial cells (HNEpCs) were used as a model of the first barrier of the respiratory system. Cell internalization and intracellular reactive oxygen species (iROS) induction, as well as the effects on mitochondria functionality and in the modulation of the autophagy pathway, were evaluated. The data indicated significant cellular uptake and increased levels of iROS. Furthermore, a loss of mitochondrial membrane potential was observed in the exposed cells. Regarding the effects on the autophagy pathway, PETNPLs exposure significantly increases LC3-II protein expression levels. PETNPLs exposure also induced significant increases in the expression of p62. This is the first study showing that true-to-life PETNPLs can alter the autophagy pathway in HNEpCs
AB - Since inhalation is a relevant exposure route, studies using appropriate micro/nanoplastic (MNPLs) models, representative targeted cells, and relevant biomarkers of effect are required. We have used lab-made polyethylene terephthalate (PET)NPLs obtained from PET plastic water bottles. Human primary nasal epithelial cells (HNEpCs) were used as a model of the first barrier of the respiratory system. Cell internalization and intracellular reactive oxygen species (iROS) induction, as well as the effects on mitochondria functionality and in the modulation of the autophagy pathway, were evaluated. The data indicated significant cellular uptake and increased levels of iROS. Furthermore, a loss of mitochondrial membrane potential was observed in the exposed cells. Regarding the effects on the autophagy pathway, PETNPLs exposure significantly increases LC3-II protein expression levels. PETNPLs exposure also induced significant increases in the expression of p62. This is the first study showing that true-to-life PETNPLs can alter the autophagy pathway in HNEpCs
KW - Autophagy
KW - HNEpCs
KW - Mitochondrial membrane potential
KW - Nanoplastics
KW - Oxidative stress
KW - PET
KW - Uptake
UR - http://www.scopus.com/inward/record.url?scp=85154608175&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/7d621394-ff60-3492-a444-d4204e7dea8b/
U2 - https://doi.org/10.1016/j.etap.2023.104140
DO - https://doi.org/10.1016/j.etap.2023.104140
M3 - Article
VL - 100
M1 - 104140
ER -