Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase

Ll Bancells; D. Balsa; N. Gómez; M. Unzeta

doi:https://doi.org/10.1016/0006-2952(87)90528-4

Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase

Ll Bancells, D. Balsa, N. Gómez, M. Unzeta

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

It has been reported that monoamine oxidase (MAO) activity (EC1.4.3.4) and, in general, enzymes possessing cationic substrates, were activated and inhibited by anionic and cationic detergents, respectively. In order to examine this hypothesis, the effect of the zwitterionic detergent CHAPS 3-[(3-cholamido propyl) dimethyl ammonio]-l-propanesulphonate was studied in comparison with the effects of cationic, anionic, and non-ionic detergents. The non-denaturating zwitterionic detergent CHAPS was used to solubilise rat liver monoamine oxidase MAO (EC1.4.3.4) of mitochondrial and microsomal origin; the solubilisation conditions, purification, inhibition and kinetic studies were then determined. These results are compared with those previously obtained with the non-ionic detergent Triton X-100, which would also be expected to have no net charge, and are interpreted in terms of specific ionic effects. © 1987.

Original language	English
Pages (from-to)	2539-2546
Journal	Biochemical Pharmacology
Volume	36
DOIs	https://doi.org/10.1016/0006-2952(87)90528-4
Publication status	Published - 1 Aug 1987

Access to Document

https://doi.org/10.1016/0006-2952(87)90528-4

Fingerprint Dive into the research topics of 'Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase'. Together they form a unique fingerprint.

Cite this

@article{9a304422b34a4030aba3ca4fc5aa15ee,

title = "Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase",

abstract = "It has been reported that monoamine oxidase (MAO) activity (EC1.4.3.4) and, in general, enzymes possessing cationic substrates, were activated and inhibited by anionic and cationic detergents, respectively. In order to examine this hypothesis, the effect of the zwitterionic detergent CHAPS 3-[(3-cholamido propyl) dimethyl ammonio]-l-propanesulphonate was studied in comparison with the effects of cationic, anionic, and non-ionic detergents. The non-denaturating zwitterionic detergent CHAPS was used to solubilise rat liver monoamine oxidase MAO (EC1.4.3.4) of mitochondrial and microsomal origin; the solubilisation conditions, purification, inhibition and kinetic studies were then determined. These results are compared with those previously obtained with the non-ionic detergent Triton X-100, which would also be expected to have no net charge, and are interpreted in terms of specific ionic effects. {\textcopyright} 1987.",

author = "Ll Bancells and D. Balsa and N. G{\'o}mez and M. Unzeta",

year = "1987",

month = aug,

day = "1",

doi = "https://doi.org/10.1016/0006-2952(87)90528-4",

language = "English",

volume = "36",

pages = "2539--2546",

}

Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase. / Bancells, Ll; Balsa, D.; Gómez, N.; Unzeta, M.

In: Biochemical Pharmacology, Vol. 36, 01.08.1987, p. 2539-2546.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Effects of nondenaturating zwitterionic detergent chaps, 3-[(3-cholamido propyl) dimethyl ammonio]-1-propanesulfonate, on rat liver mitochondrial and microsomal monoamine oxidase

AU - Bancells, Ll

AU - Balsa, D.

AU - Gómez, N.

AU - Unzeta, M.

PY - 1987/8/1

Y1 - 1987/8/1

N2 - It has been reported that monoamine oxidase (MAO) activity (EC1.4.3.4) and, in general, enzymes possessing cationic substrates, were activated and inhibited by anionic and cationic detergents, respectively. In order to examine this hypothesis, the effect of the zwitterionic detergent CHAPS 3-[(3-cholamido propyl) dimethyl ammonio]-l-propanesulphonate was studied in comparison with the effects of cationic, anionic, and non-ionic detergents. The non-denaturating zwitterionic detergent CHAPS was used to solubilise rat liver monoamine oxidase MAO (EC1.4.3.4) of mitochondrial and microsomal origin; the solubilisation conditions, purification, inhibition and kinetic studies were then determined. These results are compared with those previously obtained with the non-ionic detergent Triton X-100, which would also be expected to have no net charge, and are interpreted in terms of specific ionic effects. © 1987.

AB - It has been reported that monoamine oxidase (MAO) activity (EC1.4.3.4) and, in general, enzymes possessing cationic substrates, were activated and inhibited by anionic and cationic detergents, respectively. In order to examine this hypothesis, the effect of the zwitterionic detergent CHAPS 3-[(3-cholamido propyl) dimethyl ammonio]-l-propanesulphonate was studied in comparison with the effects of cationic, anionic, and non-ionic detergents. The non-denaturating zwitterionic detergent CHAPS was used to solubilise rat liver monoamine oxidase MAO (EC1.4.3.4) of mitochondrial and microsomal origin; the solubilisation conditions, purification, inhibition and kinetic studies were then determined. These results are compared with those previously obtained with the non-ionic detergent Triton X-100, which would also be expected to have no net charge, and are interpreted in terms of specific ionic effects. © 1987.

U2 - https://doi.org/10.1016/0006-2952(87)90528-4

DO - https://doi.org/10.1016/0006-2952(87)90528-4

M3 - Article

VL - 36

SP - 2539

EP - 2546

ER -