Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats

Sandra Soto-Heras, Maria Gracia Catalá, Montserrat Roura, Irene Menéndez-Blanco, Anna Rita Piras, Dolors Izquierdo, Maria Teresa Paramio*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Contents Melatonin enhances in vitro embryo development in several species by improving the oocyte developmental competence during in vitro maturation (IVM). Melatonin has a wide range of actions, from scavenging reactive oxygen species (ROS) to regulating gene expression, and it can also act by way of melatonin receptors. The aim of this study was to determine the mechanism of action of melatonin during the IVM of juvenile goat oocytes and the role of the membrane receptors. Melatonin receptor 1 was immunolocalized in cumulus cells and oocytes before and after 24hr of IVM. The effect of melatonin on oocyte developmental competence was tested in three experimental IVM groups: (a) control, (b) 10(-7)M melatonin, and (c) 10(-7)M melatonin +10(-7)M luzindole (an inhibitor of both melatonin receptors). After IVM oocytes were assessed for ROS levels, mitochondrial activity, adenosine 5-triphosphate (ATP) concentration and relative gene expression (ACTB, SLC1A1, SOD1, GPx1, BAX, DNMT1, GCLC and GDF9). IVM-oocytes were in vitro fertilized and cultured under conventional conditions. Blastocyst rate and quality (differential cell count) were assessed at 8days post-fertilization. Melatonin decreased ROS levels, increased mitochondrial activity and ATP content and increased blastocyst quality compared to control group (55.8 vs. 30.4 inner cell mass ICM, p

Original languageEnglish
Pages (from-to)381-390
Number of pages10
JournalReproduction in Domestic Animals
Volume54
Issue number2
DOIs
Publication statusPublished - 1 Feb 2019

Keywords

  • embryonic development
  • melatonin
  • melatonin receptors
  • mitochondria
  • oocyte maturation
  • reactive oxygen species
  • Reactive Oxygen Species/metabolism
  • Mitochondria/metabolism
  • Receptor, Melatonin, MT1/metabolism
  • Oogenesis/drug effects
  • Goats/physiology
  • Oocytes/drug effects
  • Animals
  • Cumulus Cells/drug effects
  • Female
  • In Vitro Oocyte Maturation Techniques/veterinary
  • Embryonic Development/drug effects
  • Melatonin/pharmacology
  • Blastocyst/drug effects
  • DNA-DAMAGE
  • FOLLICULAR-FLUID
  • IN-VITRO
  • GENE-EXPRESSION
  • OXIDATIVE STRESS
  • MATURATION
  • PROTECTS
  • EMBRYO DEVELOPMENT

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