TY - JOUR
T1 - Effects of etamsylate on equine platelets: In vitro and in vivo studies
AU - Segura, Dídac
AU - Monreal, Luis
AU - Pérez-Pujol, Sílvia
AU - Alonso, Antonio
AU - Díaz-Ricart, Maribel
AU - Brugués, Rosa
AU - Ordinas, Antonio
AU - Escolar, Ginés
PY - 2007/9/1
Y1 - 2007/9/1
N2 - The aim of this study was to investigate whether etamsylate produces equine platelet activation. In vitro and in vivo studies were designed in which seven and eight adult healthy horses were included, respectively. In the in vitro study, citrated blood was incubated with different concentrations of etamsylate, and P-selectin expression and annexin V binding were determined by flow cytometry. In the in vivo study, blood was collected before and 1 and 2 h after IV administration of etamsylate, and P-selectin expression was evaluated. In the in vitro study, a significant increase in P-selectin expression, leukocyte-platelet aggregate formation and annexin V binding were observed. In the in vivo study, a marked increase in P-selectin expression and heterotypic aggregate formation was seen in two and five horses, respectively, although no significant differences were detected when analyzing results from all the animals together. The results of the in vitro study indicate that etamsylate produces a pre-activation state in equine platelets, but this fact could be confirmed by the in vivo study. © 2006 Elsevier Ltd. All rights reserved.
AB - The aim of this study was to investigate whether etamsylate produces equine platelet activation. In vitro and in vivo studies were designed in which seven and eight adult healthy horses were included, respectively. In the in vitro study, citrated blood was incubated with different concentrations of etamsylate, and P-selectin expression and annexin V binding were determined by flow cytometry. In the in vivo study, blood was collected before and 1 and 2 h after IV administration of etamsylate, and P-selectin expression was evaluated. In the in vitro study, a significant increase in P-selectin expression, leukocyte-platelet aggregate formation and annexin V binding were observed. In the in vivo study, a marked increase in P-selectin expression and heterotypic aggregate formation was seen in two and five horses, respectively, although no significant differences were detected when analyzing results from all the animals together. The results of the in vitro study indicate that etamsylate produces a pre-activation state in equine platelets, but this fact could be confirmed by the in vivo study. © 2006 Elsevier Ltd. All rights reserved.
KW - Etamsylate
KW - Flow cytometry
KW - Horse
KW - P-selectin
KW - Platelet activation
U2 - https://doi.org/10.1016/j.tvjl.2006.06.002
DO - https://doi.org/10.1016/j.tvjl.2006.06.002
M3 - Article
VL - 174
SP - 325
EP - 329
ER -