Effects of different concentrations of Pseudomonas aeruginosa on boar sperm quality

Lilian Sepúlveda, Eva Bussalleu, Marc Yeste, Sergi Bonet

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    21 Citations (Scopus)

    Abstract

    © 2014 Elsevier B.V. Bacteriospermia in boar ejaculates is a frequent finding that compromises the sperm quality and, consequently, causes economic losses in swine industry. The present study sought to evaluate the effect of different concentrations of Pseudomonas aeruginosa on boar sperm quality over a storing period of 11 days at 15-17°C. Ten commercial seminal doses coming from post-pubertal and healthy boars were artificially inoculated with different infective concentrations of P. aeruginosa, ranging from 2×108 to 2×104cfu/mL. Negative controls were non-inoculated doses. Sperm quality, assessed as sperm motility (CASA), sperm viability, acrosome integrity and pH, as well as the bacterial growth, were checked after 0, 1, 2, 4, 7, 9 and 11 days of storage at 15-17°C. Results obtained showed significant decreases in the percentages of total and progressive sperm motility, sperm viability and acrosome integrity in the greatest infective concentrations (2×107 and 2×108cfu/mL), when compared to the negative control. In contrast, there was no effect on seminal pH throughout the experiment. Results indicate the presence of P. aeruginosa in boar semen, apart from being a potential source for the spread of infectious diseases and harmful impact on sows, negatively affects the longevity and fertilizing ability of boar sperm when present in high concentrations. Thus, P. aeruginosa causes deleterious effects on boar sperm quality during liquid storage at 15-17°C, thus strict hygienic measures must be implemented in boar studs to minimize bacterial concentration of semen doses.
    Original languageEnglish
    Pages (from-to)96-106
    JournalAnimal Reproduction Science
    Volume150
    Issue number3-4
    DOIs
    Publication statusPublished - 1 Jan 2014

    Keywords

    • Bacteria
    • Boar spermatozoa
    • Pseudomonas aeruginosa
    • Sperm quality

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