TY - JOUR
T1 - Effect of the surface charge of artificial model membranes on the aggregation of amyloid β-peptide
AU - Sabaté, Raimon
AU - Espargaró, Alba
AU - Barbosa-Barros, Lucyanna
AU - Ventura, Salvador
AU - Estelrich, Joan
PY - 2012/8/1
Y1 - 2012/8/1
N2 - The neurotoxicity effect of the β-amyloid (Aβ) peptide, the primary constituent of senile plaques in Alzheimer's disease, occurs through interactions with neuronal membranes. Here, we attempt to clarify the mechanisms and consequences of the interaction of Aβ with lipid membranes. We have used liposomes as a model of biological membrane, and have devoted particular attention to the bilayer charge effect. Our results show that insertion and surface association of peptide with membrane, increased in a membrane charge-dependent manner, lead to a reduction of Aβ soluble species, lag time elongation and an increase in the inter-molecular β-sheet ratio of amyloid fibrils. In addition, our findings suggest that the fine balance between peptide insertion and surface association modulates Aβ aggregation, influencing the amyloid fibrils concentration as well as their morphology. © 2012 Elsevier Masson SAS. All rights reserved.
AB - The neurotoxicity effect of the β-amyloid (Aβ) peptide, the primary constituent of senile plaques in Alzheimer's disease, occurs through interactions with neuronal membranes. Here, we attempt to clarify the mechanisms and consequences of the interaction of Aβ with lipid membranes. We have used liposomes as a model of biological membrane, and have devoted particular attention to the bilayer charge effect. Our results show that insertion and surface association of peptide with membrane, increased in a membrane charge-dependent manner, lead to a reduction of Aβ soluble species, lag time elongation and an increase in the inter-molecular β-sheet ratio of amyloid fibrils. In addition, our findings suggest that the fine balance between peptide insertion and surface association modulates Aβ aggregation, influencing the amyloid fibrils concentration as well as their morphology. © 2012 Elsevier Masson SAS. All rights reserved.
KW - Aggregation
KW - Amyloid
KW - Fibrils
KW - Liposome
KW - Model membrane
KW - Oligomer
U2 - https://doi.org/10.1016/j.biochi.2012.03.027
DO - https://doi.org/10.1016/j.biochi.2012.03.027
M3 - Article
VL - 94
SP - 1730
EP - 1738
ER -