TY - JOUR
T1 - Effect of season on intrafollicular fatty acid concentrations and embryo production after in vitro fertilization and parthenogenic activation of prepubertal goat oocytes
AU - Catala, Maria-Gracia
AU - Roura, Montserrat
AU - Soto-Heras, Sandra
AU - Menéndez, Irene
AU - Contreras-Solis, Ignacio
AU - Paramio, Maria-Teresa
AU - Izquierdo, Dolors
PY - 2018/11/1
Y1 - 2018/11/1
N2 - © 2018 Elsevier B.V. In this study we have assessed the concentration of 13 fatty acids (FAs) in follicular fluid (FF) and tested the blastocyst development of prepubertal goat oocytes after in vitro fertilization (IVF) and parthenogenic activation (PA) during spring, summer, autumn and winter. Ovaries were collected from a local slaughterhouse and transported to the laboratory during the four seasons. Samples of FF were recovered from follicles and FAs were analyzed by gas chromatography. Oocytes were in vitro matured in TCM-199 with hormones, epidermal growth factor and 10% of fetal bovine serum for 24 h. After maturation, oocytes were inseminated with fresh semen capacitated with heparin or parthenogenically activated with ionomycin and 6-dimetylaminopurine. Zygotes from both groups were cultured in SOF medium for 8 days. The results showed that the polyunsaturated FA (PUFA) concentration of n3 was significantly reduced in autumn compared to winter (46.17 μM and 116.45 μM, respectively) and the n6 to n3 (n6:n3) PUFA ratio was significantly higher in autumn than in winter (11.17 μM vs 4.23 μM, respectively). Blastocyst production was significantly higher (P < 0.05) in winter than autumn after IVF (16.8% and 5.5%, respectively) and showed a statistical tendency (P < 0.1) after PA (22.7% and 11.5%, respectively). In conclusion, in ovaries of prepubertal goats we have observed higher concentrations of n3 PUFAs and lower n6:n3 PUFA ratio in the follicular fluid and oocytes with higher competence to develop up to blastocyst in winter compared to autumn. Moreover, spring, which is a non-breeding season in goat, have not affected in vitro blastocyst production from suckling kid oocytes.
AB - © 2018 Elsevier B.V. In this study we have assessed the concentration of 13 fatty acids (FAs) in follicular fluid (FF) and tested the blastocyst development of prepubertal goat oocytes after in vitro fertilization (IVF) and parthenogenic activation (PA) during spring, summer, autumn and winter. Ovaries were collected from a local slaughterhouse and transported to the laboratory during the four seasons. Samples of FF were recovered from follicles and FAs were analyzed by gas chromatography. Oocytes were in vitro matured in TCM-199 with hormones, epidermal growth factor and 10% of fetal bovine serum for 24 h. After maturation, oocytes were inseminated with fresh semen capacitated with heparin or parthenogenically activated with ionomycin and 6-dimetylaminopurine. Zygotes from both groups were cultured in SOF medium for 8 days. The results showed that the polyunsaturated FA (PUFA) concentration of n3 was significantly reduced in autumn compared to winter (46.17 μM and 116.45 μM, respectively) and the n6 to n3 (n6:n3) PUFA ratio was significantly higher in autumn than in winter (11.17 μM vs 4.23 μM, respectively). Blastocyst production was significantly higher (P < 0.05) in winter than autumn after IVF (16.8% and 5.5%, respectively) and showed a statistical tendency (P < 0.1) after PA (22.7% and 11.5%, respectively). In conclusion, in ovaries of prepubertal goats we have observed higher concentrations of n3 PUFAs and lower n6:n3 PUFA ratio in the follicular fluid and oocytes with higher competence to develop up to blastocyst in winter compared to autumn. Moreover, spring, which is a non-breeding season in goat, have not affected in vitro blastocyst production from suckling kid oocytes.
KW - Fatty acid
KW - Follicular fluid
KW - IVF
KW - Juvenile goat
KW - Parthenogenetic activation
KW - Season
U2 - 10.1016/j.smallrumres.2018.10.003
DO - 10.1016/j.smallrumres.2018.10.003
M3 - Article
VL - 168
SP - 82
EP - 86
JO - Small Ruminant Research
JF - Small Ruminant Research
SN - 0921-4488
ER -