Effect of nitrogen sources in batch and continuous cultures to lipase production by Candida rugosa

J. L. Montesinos, N. Obradors, M. A. Gordillo, F. Valero, J. Lafuente, C. Solà

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    21 Citations (Scopus)

    Abstract

    The interaction between medium components, microorganisms and the operational mode to produce lipase by Candida rugosa was studied. It has been observed as an emulsion formation between oleic acid and urea during continuous culture. Because of this emulsion, the nitrogen source cannot be assimilated by the cells. The lack of nitrogen source provoked a decrease in protein synthesis, being lipase synthesis suppressed. The change of the nitrogen source from urea to ammonium sulfate avoided the emulsion formation, allowing to reach biomass and lipase steady states in continuous cultures. In batch cultures, no effect of nitrogen source on lipase production and biomass was observed. The specific productivity in continuous culture was slightly higher than in batch cultures. © Copyright 1996 by Humana Press Inc. All rights of any nature whatsoever reserved.
    Original languageEnglish
    Pages (from-to)25-37
    JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
    Volume59
    DOIs
    Publication statusPublished - 1 Jan 1996

    Keywords

    • Ammonium sulfate
    • Batch and continuous fermentation
    • Candida rugosa
    • Lipase production
    • Nitrogen sources
    • Oleic acid
    • Urea

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