Effect of follicle diameter on oocyte apoptosis, embryo development and chromosomal ploidy in prepubertal goats

R. Romaguera, A. Casanovas, R. Morató, D. Izquierdo, M. Catalá, A. R. Jimenez-Macedo, T. Mogas, M. T. Paramio

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25 Citations (Scopus)

Abstract

The aim of this study was to assess the following parameters in prepubertal goat oocytes of different follicle diameter (3 mm, <3 mm, control): oocyte diameter, early (Annexin-V) and late (TUNEL) apoptosis, embryo development and chromosomal ploidy of these blastocysts using Fluorescence In Situ Hybridization (FISH). Before in vitro maturation, oocytes were measured and stained with Annexin-V or TUNEL. The rest of the oocytes were matured, fertilized, and cultured in vitro for 8 days. Oocytes from follicles of 3 mm showed greater mean oocyte diameter (128.27 7.20 μm vs. 125.35 7.59 μm), higher percentages of TUNEL positive (42.86 vs. 24.23%), higher cleavage (47.85 3.98 vs. 23.07 2.44 %) and blastocyst rates (19.77 3.04 vs. 4.11 1.10 %) than oocytes from follicles of <3 mm.. Blastocyst mean cell numbers did not show differences between follicular groups (123.83 49.62 vs. 104.29 36.09 for follicles of 3 mm and <3 mm, respectively). A total of 54 blastocysts with 7084 nuclei were hybridized with specific probes to chromosomes X and Y. Ninety-eight percent (98%) of the embryos presented at least one cell carrying an abnormal number of chromosomes, but 78% of them presented less than 25% of chromosomal abnormal cells. No differences in the percentage of blastocysts with abnormal ploidy were found in embryos produced from oocytes of different follicle diameter. © 2010 Elsevier Inc.
Original languageEnglish
Pages (from-to)364-373
JournalTheriogenology
Volume74
Issue number3
DOIs
Publication statusPublished - 1 Aug 2010

Keywords

  • Apoptosis
  • Blastocyst
  • Chromosomes
  • Follicle diameter
  • Goat
  • Oocyte

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