Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

Antoni Sánchez, Rosa Maria De La Casa, Jose Vicente Sinisterra, Francisco Valero, Jose Maria Sánchez-Montero

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    11 Citations (Scopus)

    Abstract

    Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.
    Original languageEnglish
    Pages (from-to)65-75
    JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
    Volume80
    DOIs
    Publication statusPublished - 17 Jul 1999

    Keywords

    • Candida rugosa lipase
    • Downstream
    • Esterification
    • Isoenzymes
    • Ketoprofen
    • Lipolytic activity

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