Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

Antoni Sánchez; Rosa Maria De La Casa; Jose Vicente Sinisterra; Francisco Valero; Jose Maria Sánchez-Montero

doi:https://doi.org/10.1385/ABAB:80:1:65

Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

Antoni Sánchez, Rosa Maria De La Casa, Jose Vicente Sinisterra, Francisco Valero, Jose Maria Sánchez-Montero

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.

Original language	English
Pages (from-to)	65-75
Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume	80
DOIs	https://doi.org/10.1385/ABAB:80:1:65
Publication status	Published - 17 Jul 1999

Keywords

Candida rugosa lipase
Downstream
Esterification
Isoenzymes
Ketoprofen
Lipolytic activity

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https://doi.org/10.1385/ABAB:80:1:65

Cite this

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title = "Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa",

abstract = "Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.",

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Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa. / Sánchez, Antoni; De La Casa, Rosa Maria; Sinisterra, Jose Vicente et al.
In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 80, 17.07.1999, p. 65-75.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

AU - Sánchez, Antoni

AU - De La Casa, Rosa Maria

AU - Sinisterra, Jose Vicente

AU - Valero, Francisco

AU - Sánchez-Montero, Jose Maria

PY - 1999/7/17

Y1 - 1999/7/17

N2 - Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.

AB - Different fed-batch cultures of Candida rugosa were carried out using oleic acid as the only carbon source. The crude lipases obtained under several operational conditions and downstream processes showed different catalytic activity and isoenzymes ratio. This fact implied that the performance of the lipase produced could be modulated by using different operational fermentation conditions. These powders were compared with commercial lipase from Sigma (St. Louis, MO) in hydrolysis and synthesis reactions. Especially interesting was the fact that the enantioselectivity of a crude lipase was higher than that observed with commercial lipase in the resolution of racemic Ketoprofen. In addition, response of both lipases in the presence of water was different.

KW - Candida rugosa lipase

KW - Downstream

KW - Esterification

KW - Isoenzymes

KW - Ketoprofen

KW - Lipolytic activity

U2 - https://doi.org/10.1385/ABAB:80:1:65

DO - https://doi.org/10.1385/ABAB:80:1:65

M3 - Article

SN - 0273-2289

VL - 80

SP - 65

EP - 75

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

ER -

Effect of fermentation conditions in the enzymatic activity and stereoselectivity of crude lipase from Candida rugosa

Abstract

Keywords

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