Effect of dodecyl maltoside detergent on rhodopsin stability and function

Eva Ramon, Jordi Marron, Luis Del Valle, Laia Bosch, Anna Andrés, Joan Manyosa, Pere Garriga

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)

Abstract

Detergent-solubilized bovine rhodopsin produces mixed detergent/lipid/ protein micelles. The effect of dodecyl maltoside detergent on the thermal stability of dark-state rhodopsin, and upon formation of the different intermediates after rhodopsin photobleaching (metarhodopsin II and metarhodopsin III), and upon transducin activation has been studied. No significant effect is observed for the thermal stability of dark-state rhodopsin in the range of detergent concentrations studied, but a decrease in the stability of metarhodopsin II and an increase in metarhodopsin III formation is observed with decreasing detergent concentrations. The transducin activation process is also affected by the presence of detergent indicating that this process is dependent on the lipid micro-environment and membrane fluidity, and this stresses the importance of the native lipid environment in rhodopsin normal function. © 2003 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)3055-3061
JournalVision Research
Volume43
Issue number28
DOIs
Publication statusPublished - 1 Jan 2003

Keywords

  • Dodecyl maltoside
  • G activation t
  • Metarhodopsin II
  • Metarhodopsin III
  • Rhodopsin

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