Effect of Diet Supplementation with the Mycotoxin Binder Montmorillonite on the Bioavailability of Vitamins in Dairy Cows

Abdelhacib Kihal, Cristina Marquès, María Rodríguez-Prado, Eduard Jose-Cunilleras, Sergio Calsamiglia*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)


The objective of this study was to determine the effect of the mycotoxin binder montmorillonite (MMT) supplemented in the diet of dairy cows on the bioavailability of vitamins A, D, E, B1 and B6. Six multiparous Holstein-Friesian cows were used in a crossover design with two periods. Treatments were a control diet with or without MMT. Vitamins were infused individually into the abomasum through the ruminal cannula. Blood samples were collected from the jugular vein at 0, 1, 2, 3, 4, 6, 9, 12, 24 and 48 h after the administration of each vitamin. Results showed that vitamin A reached maximal concentration (Tmax) at 5.3 h after dosing, the maximal concentration (Cmax) was 1.2 times higher than the basal concentration (Cbasal), and the area under the curve (AUC) was 739 arbitrary units. Vitamin B6 reached the Tmax at 13 h after dosing, the Cmax was 1.4 times higher than the Cbasal, and the AUC was 222 arbitrary units. No differences were observed in Cbasal, Tmax, Cmax and AUC of vitamin A and B6 between control vs. MMT-supplemented cows. Plasma concentrations of vitamins D, E and B1 had no concentration peaks, and were not affected by MMT addition. The lack of a response suggests that their plasma concentration may be tightly regulated. Results of this study do not show evidence that MMT affects the bioavailability of vitamins A and B6 in vivo.

Original languageEnglish
Article number26
Number of pages8
Issue number1
Publication statusPublished - 1 Jan 2022


  • Adsorption
  • Animal Feed/analysis
  • Animals
  • Bentonite/metabolism
  • Binding capacity
  • Bioavailability
  • Biological Availability
  • Cattle/metabolism
  • Dietary Supplements
  • Mycotoxin binder
  • Mycotoxins/metabolism
  • Spain
  • Vitamins
  • Vitamins/blood
  • adsorption
  • binding capacity
  • bioavailability
  • mycotoxin binder
  • vitamins


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