Introduction: Chondroitin sulfate (CS) and hyaluronic acid (HA) are used in the symptomatic treatment of osteoarthritis (OA). Cholagenase-1 (MMP-1) and stromelysin-1 (MMP-3), are responsible for degradation of the extracellular matrix in OA. Few studies have determined the in vitro effect of CS and HA on MMP-1 synthesis and that of HA on MMP-3 expression in human OA chondrocyte culture. In the literature reviewed, there were no studies evaluating the effect of CS on MMP-3. Objectives: To analyze the effect of CS and HA (500-730 kDa) on MMP-3 and MMP-1 synthesis induced by interleukin-1β (IL-1β) in OA chondrocytes. Material and methods: Chondrocytes were incubated for 48 hours with IL-1β (2.5 ng/ml) in the presence or absence of different HA concentrations (Hyalgan®, Bioibérica Farma) (10, 50, 100, 150, 200 and 1000 μg/ml). Functional evaluation of chondrocytes was performed by enzyme-immunoanalysis of MMP-1 and MMP-3 levels. Results: CS and HA inhibited IL-1β-induced MMP-3 synthesis, without significantly modifying MMP-1. CS and HA reduced levels of MMP-3 expression at all the studied concentrations, with no statistically significant differences among these concentrations. Conclusions: The results of this study show for the first time that CS inhibits MMP-3 synthesis in OA cartilage. and corroborates the few existing data on the ability of HA to inhibit this enzyme. © 2005 Elsevier España S.L. Barcelona.
- Chondroitin sulfate
- Hyaluronic acid