Effect of chondroitin sulfate and hyaluronic acid (500-730 kDa) on synthesis of stromelysin (MMP-3) and MMP-1 in human chondrocyte cultures

J. Monfort, M. Nacher, E. Montell, E. Tomàs, J. Vergés, P. Benito

    Research output: Contribution to journalArticleResearchpeer-review

    2 Citations (Scopus)

    Abstract

    Introduction: Chondroitin sulfate (CS) and hyaluronic acid (HA) are used in the symptomatic treatment of osteoarthritis (OA). Cholagenase-1 (MMP-1) and stromelysin-1 (MMP-3), are responsible for degradation of the extracellular matrix in OA. Few studies have determined the in vitro effect of CS and HA on MMP-1 synthesis and that of HA on MMP-3 expression in human OA chondrocyte culture. In the literature reviewed, there were no studies evaluating the effect of CS on MMP-3. Objectives: To analyze the effect of CS and HA (500-730 kDa) on MMP-3 and MMP-1 synthesis induced by interleukin-1β (IL-1β) in OA chondrocytes. Material and methods: Chondrocytes were incubated for 48 hours with IL-1β (2.5 ng/ml) in the presence or absence of different HA concentrations (Hyalgan®, Bioibérica Farma) (10, 50, 100, 150, 200 and 1000 μg/ml). Functional evaluation of chondrocytes was performed by enzyme-immunoanalysis of MMP-1 and MMP-3 levels. Results: CS and HA inhibited IL-1β-induced MMP-3 synthesis, without significantly modifying MMP-1. CS and HA reduced levels of MMP-3 expression at all the studied concentrations, with no statistically significant differences among these concentrations. Conclusions: The results of this study show for the first time that CS inhibits MMP-3 synthesis in OA cartilage. and corroborates the few existing data on the ability of HA to inhibit this enzyme. © 2005 Elsevier España S.L. Barcelona.
    Original languageEnglish
    Pages (from-to)150-154
    JournalReumatologia Clinica
    Volume1
    DOIs
    Publication statusPublished - 1 Oct 2005

    Keywords

    • Chondroitin sulfate
    • Hyaluronic acid
    • Metalloproteases
    • Osteoarthritis

    Fingerprint

    Dive into the research topics of 'Effect of chondroitin sulfate and hyaluronic acid (500-730 kDa) on synthesis of stromelysin (MMP-3) and MMP-1 in human chondrocyte cultures'. Together they form a unique fingerprint.

    Cite this