Effect of acyl-acceptor stepwise addition strategy using alperujo oil as a substrate in enzymatic biodiesel synthesis

Kírian Bonet-Ragel, Albert Canet, M. Dolors Benaiges, Francisco Valero

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14 Citations (Scopus)


© 2017 Society of Chemical Industry BACKGROUND: Using renewable feedstock sources for biodiesel production seems to be a promising strategy and even more so when enzymatic catalysis with lipases are used. However, it is well known that these enzymes could be inactivated by reaction conditions such as temperature or alcohol concentration. In this work, the effect of temperature and initial water activity (aw) value on immobilised recombinant Rhizopus oryzae lipase (rROL) were studied. Methanolysis and ethanolysis reactions using alperujo oil with three different stepwise addition strategies were employed. RESULTS: Recombinant 1,3-positional selective rROL covalently immobilised on polymethacrylate amino-epoxy activated support showed maximum initial reaction rate at low aw value (0.093). It was found that 30 °C was the optimal temperature in terms of biocatalyst stability during transesterification reactions. Adding alcohol at once, ethanol was clearly a better acyl-acceptor in terms of stability than methanol. Productivity was found to be 2-fold higher when five pulses of ethanol were used instead of methanol. CONCLUSIONS: Alperujo oil has great potential as a low cost feedstock for biodiesel production through enzymatic catalysis using a nearly semi-continuous alcohol addition strategy. © 2017 Society of Chemical Industry.
Original languageEnglish
Pages (from-to)541-547
JournalJournal of Chemical Technology and Biotechnology
Issue number2
Publication statusPublished - 1 Feb 2018


  • Rhizopus oryzae lipase
  • biodiesel synthesis
  • ethanolysis
  • methanolysis
  • waste oil source
  • water activity


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