Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI

Rui V. Simões, Juan E. Ortuño, Louisa Bokacheva, Ana P. Candiota, Maria J. Ledesma-Carbayo, Teresa Delgado-Goñi, Maria L. García-Martín, Andrés Santos, Carles Arús

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Abstract

© 2014, ESMRMB. Objective: We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C).Materials and methods: Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI before and after an injection of glucose (n = 4) or saline (n = 3). Maximum relative contrast enhancement (RCE) and initial area under the enhancement curve (IAUC) were determined in each pixel.Results: The mean tumor parameter values showed no significant changes after injecting either saline (RCE −5.9 ± 5.0 %; IAUC −3.7 ± 3.6 %) or glucose (RCE −1.6 ± 9.0 %; IAUC +0.6 ± 6.4 %). Pixel-by-pixel analysis revealed small post-injection changes in RCE and IAUC between the glucose and saline groups, all within 13 % range of their baseline values.Conclusion: Perturbing the metabolism of GL261 tumors kept at moderate hypothermia with hyperglycemia did not induce significant changes in the permeability/perfusion of these tumors. This is relevant for future studies with this model since regional differences in glucose accumulation could thus reflect basal heterogeneities in vasculature and/or metabolism of GL261 tumors.
Original languageEnglish
Pages (from-to)119-126
JournalMagnetic Resonance Materials in Physics, Biology and Medicine
Volume28
Issue number2
DOIs
Publication statusPublished - 1 Apr 2015

Keywords

  • Acute hyperglycemia
  • Brain tumor
  • Contrast agent
  • GL261 cells
  • Model-free quantification
  • Moderate hypothermia
  • Perfusion
  • Permeability

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    Simões, R. V., Ortuño, J. E., Bokacheva, L., Candiota, A. P., Ledesma-Carbayo, M. J., Delgado-Goñi, T., García-Martín, M. L., Santos, A., & Arús, C. (2015). Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI. Magnetic Resonance Materials in Physics, Biology and Medicine, 28(2), 119-126. https://doi.org/10.1007/s10334-014-0447-2