Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

Elena Ibañez; Francesca Vidal; Juan Hidalgo Pareja

doi:10.1017/S0967199400002410

Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

Elena Ibañez, Francesca Vidal, Juan Hidalgo Pareja^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Polyclonal antibodies that cross-react with rodent metallothionein I (MTI) and metallothionein II (MTII) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes. © 1995, Trustees of Princeton University. All rights reserved.

Original language	English
Pages (from-to)	81-84
Journal	Zygote
Volume	3
DOIs	https://doi.org/10.1017/S0967199400002410
Publication status	Published - 1 Jan 1995

Keywords

Embryo development
Metallothionein
Microinjection
Mouse

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10.1017/S0967199400002410

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title = "Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies",

abstract = "Polyclonal antibodies that cross-react with rodent metallothionein I (MTI) and metallothionein II (MTII) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes. {\textcopyright} 1995, Trustees of Princeton University. All rights reserved.",

keywords = "Embryo development, Metallothionein, Microinjection, Mouse",

author = "Elena Iba{\~n}ez and Francesca Vidal and {Hidalgo Pareja}, Juan",

year = "1995",

month = jan,

day = "1",

doi = "10.1017/S0967199400002410",

language = "English",

volume = "3",

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journal = "Zygote",

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T1 - Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

AU - Ibañez, Elena

AU - Vidal, Francesca

AU - Hidalgo Pareja, Juan

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Polyclonal antibodies that cross-react with rodent metallothionein I (MTI) and metallothionein II (MTII) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes. © 1995, Trustees of Princeton University. All rights reserved.

AB - Polyclonal antibodies that cross-react with rodent metallothionein I (MTI) and metallothionein II (MTII) were microinjected in 1-cell and 2-cell mouse embryos, into either the cytoplasm or the nucleus. Regardless of the experimental treatment, mouse embryo development in vitro was not affected and most of the embryos cleaved normally until the morula stage. The results suggest that metallothionein is not essential for normal mouse early preimplantational development, in agreement with recent studies in mice with inactivated MT I and MT II genes. © 1995, Trustees of Princeton University. All rights reserved.

KW - Embryo development

KW - Metallothionein

KW - Microinjection

KW - Mouse

U2 - 10.1017/S0967199400002410

DO - 10.1017/S0967199400002410

M3 - Article

SN - 0967-1994

VL - 3

SP - 81

EP - 84

JO - Zygote

JF - Zygote

ER -

Early mouse preimplantation development is unaffected by microinjection of metallothionein antibodies

Abstract

Keywords

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