Early Macrophage Infiltration and Sustained Inflammation in Kidneys From Deceased Donors Are Associated With Long-Term Renal Function

E. Guillén-Gómez, I. Dasilva, I. Silva, Y. Arce, C. Facundo, E. Ars, A. Breda, A. Ortiz, L. Guirado, J. A. Ballarín, M. M. Díaz-Encarnación

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

© Copyright 2016 The American Society of Transplantation and the American Society of Transplant Surgeons Kidney transplants from living donors (LDs) have a better outcome than those from deceased donors (DDs). Different factors have been suggested to justify the different outcome. In this study, we analyzed the infiltration and phenotype of monocytes/macrophages and the expression of inflammatory and fibrotic markers in renal biopsy specimens from 94 kidney recipients (60 DDs and 34 LDs) at baseline and 4 months after transplantation. We evaluated their association with medium- and long-term renal function. At baseline, inflammatory gene expression was higher in DDs than in LDs. These results were confirmed by the high number of CD68-positive cells in DD kidneys, which correlated negatively with long-term renal function. Expression of the fibrotic markers vimentin, fibronectin, and α–smooth muscle actin was more elevated in biopsy specimens from DDs at 4 months than in those from LDs. Gene expression of inflammatory and fibrotic markers at 4 months and difference between 4 months and baseline correlated negatively with medium- and long-term renal function in DDs. Multivariate analysis point to transforming growth factor-β1 as the best predictor of long-term renal function in DDs. We conclude that early macrophage infiltration, sustained inflammation, and transforming growth factor-β1 expression, at least for the first 4 months, contribute significantly to the difference in DD and LD transplant outcome.
Original languageEnglish
Pages (from-to)733-743
JournalAmerican Journal of Transplantation
Volume17
Issue number3
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • basic (laboratory) research/science
  • biopsy
  • donors and donation
  • fibrosis
  • graft survival
  • kidney (allograft) function/dysfunction
  • kidney transplantation/nephrology
  • molecular biology
  • translational research/science

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