Early determination of cystic fibrosis by electrochemical chloride quantification in sweat

Javier Gonzalo-Ruiz, Roser Mas, Carmen de Haro, Enric Cabruja, Rafael Camero, M. Asuncion Alonso-Lomillo, F. Javier Muñoz

    Research output: Contribution to journalArticleResearchpeer-review

    63 Citations (Scopus)

    Abstract

    A novel and rapid approach to quantify chloride concentration in sweat for early detection of cystic fibrosis (CF) is shown in this work. Disposable screen-printed sensor (SPS) devices capable to induce sweat and measure the chloride concentration are presented. Pilocarpine, which was forced into de skin by means of iontophoresis, has been used to stimulate the sweat glands. Chloride concentration has been directly measured on the skin by potentiometry. The performance of the devices has been tested in synthetic samples, obtaining good agreement with the Nernst equation. Sensors reproducibility has been analyzed in terms of residual standard deviation (RSD), obtaining a value of 8% (n = 6 and α = 0.05). Finally, the application of these sensors in several volunteers has been carried out. The results were compared with the method generally used in hospitals, obtaining deviations minor than 8%. © 2008 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)1788-1791
    JournalBiosensors and Bioelectronics
    Volume24
    Issue number6
    DOIs
    Publication statusPublished - 15 Feb 2009

    Keywords

    • Biomedical device
    • Cystic fibrosis
    • Potentiometric sensor
    • Screen-printed chloride electrodes
    • Sweat test

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