Dynamic Imaging of Cell-Free and Cell-Associated Viral Capture in Mature Dendritic Cells

Nuria Izquierdo-Useros, Olga Esteban, Maria T. Rodriguez-Plata, Itziar Erkizia, Julia G. Prado, Julià Blanco, Maria F. García-Parajo, Javier Martinez-Picado

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Dendritic cells (DCs) capture human immunodeficiency virus (HIV) through a non-fusogenic mechanism that enables viral transmission to CD4 + T cells, contributing to in vivo viral dissemination. Although previous studies have provided important clues to cell-free viral capture by mature DCs (mDCs), dynamic and kinetic insight on this process is still missing. Here, we used three-dimensional video microscopy and single-particle tracking approaches to dynamically dissect both cell-free and cell-associated viral capture by living mDCs. We show that cell-free virus capture by mDCs operates through three sequential phases: virus binding through specific determinants expressed in the viral particle, polarized or directional movements toward concrete regions of the cell membrane and virus accumulation in a sac-like structure where trapped viral particles display a hindered diffusive behavior. Moreover, real-time imaging of cell-associated viral transfer to mDCs showed a similar dynamics to that exhibited by cell-free virus endocytosis leading to viral accumulation in compartments. However, cell-associated HIV type 1 transfer to mDCs was the most effective pathway, boosted throughout enhanced cellular contacts with infected CD4 + T cells. Our results suggest that in lymphoid tissues, mDC viral uptake could occur either by encountering cell-free or cell-associated virus produced by infected cells generating the perfect scenario to promote HIV pathogenesis and impact disease progression. © 2011 John Wiley & Sons A/S.
Original languageEnglish
Pages (from-to)1702-1713
JournalTraffic
Volume12
Issue number12
DOIs
Publication statusPublished - 1 Dec 2011

Keywords

  • Dendritic cells
  • HIV-1
  • Live cell imaging
  • Trans-infection

Fingerprint

Dive into the research topics of 'Dynamic Imaging of Cell-Free and Cell-Associated Viral Capture in Mature Dendritic Cells'. Together they form a unique fingerprint.

Cite this